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SUMMARYExpression of CAM PATH-1H. a hutnanized MoAb directed against an abundant surface antigen on human lymphocytes, has been studied using transfected tnycloma cells. As the site of chromosome integration of DNA transfected into a cell is random we investigated the feasibility and frequency of hitting a'jackpot'site for expression after co-transfection with CAMPATH-IH heavy and light chain constructs in genomic context. A cell line generating 40 ^ig/ml of CAMPATH-IH in spent culture supernatant was achieved. The full nucleotide sequence of the CAMPATH-IH heavy and light chain cDNA clones is also shown, in addition a comparison of the efleclor mechanisms, antibody dependent cellular cytotoxicity and complement dependent cytotoxicity. of myeloma cell and Chinese hamster ovary (CHO) cell-derived CAMPATH-IH is reported.

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