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The antidiarrhoeic marker of the whole plant of Euphorbia hirta, viz., quercetin-3-O-b-D-rhamnoside was quantitatively estimated over a number of samples collected from different parts of India by HPTLC. The bioactive marker was found to vary significantly (0.013-0.108%) and accumulated to a maximum in the Tirunelveli (8°40¢N 77°42¢E) sample. The effect of various controlling parameters was studied and it was found that the varying proportions of plant parts in the samples of E. hirta is responsible for variation in active marker concentration.

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