SUMMARYThe distribution of an epitope of the transferrin receptor in the human uterine cervical epithelium has been investigated. Immunohistochemical staining, both immunofluorescent and immunoperoxidase, was performed on biopsy specimens and cytological samples from normal, dysplastic, and neoplastic cervical epithelia using the monoclonal OKT9 antibody. The results of staining 145 cervical biopsy specimens with OKT9 showed widespread staining in all malignant epithelia and most severely dysplastic epithelia. No such staining was seen in either normal epithelia or in mildly dysplastic epithelia apart from the staining of the basal cell layer in some normal epithelia. The incidence of staining in the 50 cervical cytocentrifuge preparations was not as high as that in the 145 tissue sections.The potential role of the OKT9 antibody in both the screening of cervical cytocentrifuge preparations and the prediction of malignancy is discussed. The antibody is considered to be of more value in the examination of biopsy material than of cytocentrifuge preparations.The monoclonal antibody OKT9, raised against human leukaemia cells, recognises an epitope of the transferrin receptor.' This receptor is thought to have an essential role in the transport of iron across the cell membrane and has been detected on cells which have a large iron requirement such as reticulocytes and placental syncytiotrophoblast.23 In addition to this the receptor has a widespread distribution on transformed human cell lines4 and an association with cell proliferation and activation.5' The transferrin receptor has been found on breast cancer cells 9 and antitransferrin receptor antibody inhibits human tumour cell growth in mice.'0 This work on proliferating and malignant cells encouraged us to investigate the distribution of the transferrin receptor in the human uterine cervix. In this study biopsy specimens and cytological samples from normal, dysplastic, and malignant epithelia were examined using the OKT9 antibody in immunofluorescence and immunoperoxidase techniques.
Ileal pouch-anal anastomosis is the definitive therapy for ulcerative colitis that is refractory to medical treatment or that has developed neoplasia. Patients with this procedure are routinely followed using directed endoscopic biopsies to monitor for dysplasia in the rectal cuff, residual/recurrent ulcerative colitis, and nonspecific acute inflammation of the ileal pouch (pouchitis), which have different clinical management and outcomes. Thus, accurate localization of mucosal biopsies is crucial to a definitive histological diagnosis, but is complicated by overlapping clinical presentations of pouchitis and ulcerative colitis, post-surgical and inflammatory changes to the mucosa, and altered endoscopic anatomy, resulting in difficulty determining whether a mucosal biopsy is ileal or rectal in origin for both the endoscopist and the pathologist. We explored the utility of CD10 immunohistochemistry to aid diagnosis in this clinical setting by highlighting the enteric mucosa, based on previous studies showing its utility in brush border staining and in the diagnosis of microvillous inclusion disease. We found uniformly positive CD10 immunostaining in normal enteric mucosa, but variable loss of expression in the setting of active enteritis. Specifically, CD10 staining was lost in up to 10% of the mucosa in 1/12 ileostomies and 4/13 enteric anastomoses, in 10-80% of the mucosa in 9/10 cases of Crohn's ileitis, in 10-60% of the mucosa in 7/16 ileal pouches, and in 20-90% of the mucosa in 6/8 cases of backwash ileitis, usually in the presence of active inflammation. There was no CD10 expression by normal or diseased colonic mucosa. Therefore, while CD10 immunostaining identifies normal enteric mucosa with 100% specificity, negative staining does not definitively exclude small intestinal mucosa in the setting of active enteritis, a common condition in ileal pouch mucosa.
SUMMARY Immunohistochemical staining was performed on biopsies and cytological samples from normal, dysplastic and neoplastic squamous epithelia using the monoclonal Ca 1 antibody. The results of staining 92 biopsies and 20 cytological samples are described and it is reported that positive staining with Ca 1 antibody was detected in normal, dysplastic and neoplastic epithelia.The role of the Ca 1 antibody in the study of cervical cancer is discussed. immunohistochemical procedure to detect the Ca antigen in tissue sections. These workers showed that the majority of malignant tumours examined expressed the Ca antigen, whereas the only normal tissues to bind the Ca 1 antibody were the transitional epithelium of the urinary tract and the epithelium of the fallopian tube. In the present study the monoclonal Ca 1 antibody was used in immunofluorescence and immunoperoxidase procedures on fresh and fixed tissue from the cervix uteri. Carcinoma of the cervix uteri is exceptional in that preinvasive conditions can be diagnosed cytologically and can be readily biopsied. Thus biopsies and cytological samples from normal, dysplastic and invasive epithelia were examined for the presence of the Ca antigen in an attempt to investigate the relation between malignancy and the occurrence of the Ca antigen.
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