The objective of this study was to investigate the effects of a preparation from herbal extracts (PHE) on libido and semen quality in breeding artificial insemination boars. Ten fertile boars were divided into control and experimental groups according to significant difference of libido. There were no differences in semen quality between groups. Animals were fed a commercial feeding mixture for boars. The feeding mixture for the experimental group was enriched with PHE, which was prepared from Eurycoma longifolia, Tribulus terrestris and Leuzea carthamoides. Duration of the experiment was 10 weeks. Samples of ejaculate were collected weekly. Libido was evaluated according to a scale of 0-5 points. Semen volume, sperm motility, percentage of viable spermatozoa, sperm concentration, morphologically abnormal spermatozoa, daily sperm production and sperm survival were assessed. Amounts of mineral components and free amino acids were analysed in seminal plasma. Significant differences were found in these parameters: libido (4.05 ± 0.22 vs 3.48 ± 0.78; p < 0.001), semen volume (331.75 ± 61.91 vs 263.13 ± 87.17 g; p < 0.001), sperm concentration (386.25 ± 107.95 vs 487.25 ± 165.50 × 10(3) /mm(3); p < 0.01), morphologically abnormal spermatozoa (15.94 ± 11.08 vs 20.88 ± 9.19%; p < 0.001) and Mg concentration (28.36 ± 11.59 vs 20.27 ± 13.93 mm; p < 0.05). The experimental group's libido was increased by 20% in comparison with the beginning of the experiment. Results of this study showed positive effect of PHE on libido and some parameters of boar semen quality.
ABSTRACT:The aim of this study was to investigate the antibacterial activity and toxicity for sperm cells of the natural substances gallic acid, methyl gallate, ethyl gallate, propyl gallate, octyl gallate, thymol, carvacrol and eugenol. The antibacterial activity of these natural substances and selected combinations of them against bacterial strains isolated from boar ejaculates was determined using the microdilution and macrodilution method in Mueller-Hinton broth. The most effective natural substances against Gram-negative and Gram-positive bacteria included in our study were thymol and carvacrol with minimum inhibitory concentration (MIC) values in the range of 300-600 µg/ml. Gallic acid exhibited the best antibacterial activity against Pseudomonas aeruginosa strains (MIC values of 300-2400 µg/ml), whereas the ranges of MIC values against Escherichia coli, Enterococcus faecalis and Staphylococcus sp. strains were higher. Octyl gallate exhibited stronger antibacterial activity against staphylococci and enterococci (MIC values of 18.8-75 µg/ml) than against Escherichia coli and Pseudomonas aeruginosa strains with MIC values in the ranges of 300-600 µg/ml and 1200-2400 µg/ml, respectively. Thymol combined with carvacrol was the most effective combination against enterococci (MIC values of 75-300 : 150 thymol : carvacrol) and Pseudomonas aeruginosa (MIC values of 75-300 : 300 thymol : carvacrol), bacteria which are known to be frequently resistant to antimicrobials. Similar results were determined for the combination of carvacrol and eugenol against staphylococci and enterococci. The results of the combinations revealed more of an additive rather than a synergistic effect. Thymol and carvacrol were the most effective natural substances against the bacteria included in this study, with a low toxicity for sperm cells compared to other substances, suggesting their possible use for boar semen decontamination.
The objective of this study was to investigate the effect of using different extenders viz. Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed. Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.
The objective of this study was to analyze the influence of the year-season effect on semen production parameters in the fertile AI boars. The evaluation was especially focused on the morphologically abnormal spermatozoa (MAS) incidence. It was microscopically evaluated after making fresh semen smears and staining on microscopic slides. MAS incidence 19.46 % was lower at first half-year than at second half-year 25.00 % (P<0.01). Spermatozoa with distal protoplasmic droplet were furthest participated in total MAS incidence. Its rate was the highest at fourth quarter in comparison with annual period (P<0.001). We found that season has the negative effect on sperm morphology and significantly affects boar sperm quality and subsequently AI dose quality.Keywords: boar; morphologically abnormal spermatozoa; seasonal effect ABSTRAKTCílem této práce bylo zjistit vliv ročního období na kvalitu spermatu plemenných kanců se zvláštním zaměřením na výskyt a strukturu morfologických změn spermií. Abnormality spermií byly posuzovány mikroskopicky, prostřednictvím obarveného nátěru nativního spermatu na podložním sklíčku. Sezónní výskyt morfologicky abnormálních spermií lze komentovat nižším výskytem v prvém pololetí 19,46 % a vyšším výskytem ve druhém pololetí roku 25,00 % (P<0,01). Spermie s distální protoplazmatickou kapkou se podílely v největší míře na celkovém výskytu morfologicky abnormálních spermií, přičemž ve srovnání se zbývající částí roku byl jejich podíl nejvyšší ve čtvrtém čtvrtletí roku (P<0,001). Bylo shledáno, že vliv ročního období má negativní efekt na morfologii spermií a výrazně ovlivňuje kvalitu spermatu kanců a následně i kvalitu inseminační dávky. Jan LIPENSKÝ, Alena LUSTYKOVÁ, Josef ČEŘOVSKÝ DETAILED ABSTRACTCílem předložené studie bylo analyzovat vliv ročního období (sezóny roku) na dynamiku kvalitativních parametrů spermatu kanců používaných celoročně na ISK k produkci inseminačních dávek se zvláštním zaměřením na výskyt a strukturu morfologických změn spermií. U souboru kanců, chovaných v relativně optimálních podmínkách výživy a ošetřování, byly sledovány změny kvality spermatu v závislosti na sezóně roku, resp. podle čtvrtletí (I. leden -březen, II. duben -červen, III. červenec -září, IV. říjen -prosinec). Hodnocení výskytu morfologicky abnormálních spermií (MAS) probíhalo v období let 2006 až 2008, přičemž zpracováno a mikroskopicky vyhodnoceno bylo celkem 623 vzorků od 68 kanců různé plemenné příslušnosti. Posuzovány byly morfologicky abnormální změny na hlavičce spermií, bičíku, akrozómu, dále výskyt proximálních protoplazmatických kapek (PPK) a distálních protoplazmatických kapek (DPK) na bičíku a výše nezařazené jako vady ostatní. Tabulka 1 srovnává procento MAS dle sledovaných let a jejich čtvrtletí. Sezónní výskyt MAS lze komentovat nižším výskytem v prvém pololetí 19,46 % a vyšším výskytem ve druhém pololetí 25,00 % (P<0,01). Podrobná analýza podílu jednotlivých skupin vad spermií z celkového počtu MAS zjištěných v jednotlivých čtvrtletích za celé sledované období je prezentována v...
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