Metabolism studies of the antitumor drug etoposide show the formation of metabolites in the lactone ring, which are probably not important for the drug's mechanism of action, and oxidative transformations in the dimethoxyphenol ring (E ring), which lead to products that can cause DNA damage and may play a role in the drug's mechanism of action. The cytotoxicity of etoposide is caused by the induction of DNA damage. The occurrence of the DNA lesions can be explained by the capacity of the drug to interfere with the scission-reunion reaction of mammalian topoisomerase II by stabilizing a cleavable complex.

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A two-dimensional variational ambiguity removal technique (2DVAR) is presented. It first makes an analysis based on the ambiguous scatterometer wind vector solutions and a model forecast, and next selects the ambiguity closest to the analysis as solution. 2DVAR is applied on SeaWinds scatterometer data and its merits for nowcasting applications are shown in a general statistical comparison with model forecasts and buoy observations, and in a number of case studies. The sensitivity of 2DVAR to changes in the parameters of its underlying error model is studied. It is shown that observational noise in the nadir swath of SeaWinds is effectively suppressed by application of 2DVAR in combination with the multisolution scheme (MSS). MSS retains the local wind vector probability density function after inversion, rather than only a limited number of ambiguous solutions. As a consequence, the influence of the background increases, but this can be mitigated by switching off variational quality control. A case study on an extratropical cyclone of hurricane force intensity observed with SeaWinds at 25-km resolution shows that reliable wind estimates can be obtained for wind speeds up to 40 m s 21 and more. At 25 km, the results of 2DVAR with MSS compare better with buoy measurements than with the ECMWF model. At 100-km resolution this is reversed, proving that 2DVAR retrieves small-scale features absent in the ECMWF model.

Nine collaborating laboratories assayed 6 blind duplicate pairs of food samples containing the fructans inulin or oligofructose. The 6 sample pairs ranged from low (4%) to high levels (40%). Following the proposed method, the samples were treated with amyloglucosidase and inulinase enzymes and the released sugars were determined byion exchange chromatography. Repeatability standard deviation ranged from 2.9 to 5.8%; reproducibility standard deviation ranged from 4.7 to 11.1%. The ion-exchange chromatographic method for determinationof fructans in food and food products has been adopted first action by AOAC INTERNATIONAL (997.08)