Remodeling changes were seen when the entry tear was covered. The fate of the FL was determined by persistent antegrade flow and the level of the retrograde flow. Endografting for thoracic type B dissection was successful and promoted positive aortic remodeling changes.
A 100,OOO g supernatant fraction from rat brain that was passed through a column Receipient of a scholarship from the Instituto Nacional de Farmacologia y Bromatologia dependiente de la Secretaria de Salud Pliblica de la Nacion. Argentina. 97
The product of the incorporation of [14C]tyrosine as single unit into a protein of the soluble fraction of rat brain homogenate was purified by following a procedure used to purify tubulin. Sodium dodecylsulphate -polyacrylamide gel electrophoresis of the purified material showed a single protein band containing all the radioactivity. Purification data indicate that this protein accounts for 10.2 of the total protein of the supernatant fraction. This is in good agreement with the amount found for tubulin by the [3H]colchicine-binding method (10.5 % of the total protein). The incorporated ['"CItyrosine was found in the a-subunit of tubulin.
Protein labelled with [3H]colchicine and [14C]tyrosine was precipitated with vinblastine sulphateand the radioactivity of 3H and that of 14C were quantitatively recovered in the precipitate (98 %).Sodium dodecylsulphate -polyacrylamide gel electrophoresis of the vinblastine precipitate showed that the I4C radioactivity moved with the tubulin band.Results obtained in experiments with phenylalanine and 3,4-dihydroxyphenylalanine were identical to those obtained for tyrosine.Binding of colchicine did not interfere with the incorporation of tyrosine.
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