Although the human-landing catch (HLC) method is the most effective for
collecting anthropophilic anophelines, it has been increasingly abandoned,
primarily for ethical considerations. The objective of the present study was to
develop a new trap for the collection of Anopheles darlingi .
The initial trials were conducted using the BG-Sentinel trap as a standard for
further trap development based on colour, airflow direction and illumination.
The performance of the trap was then compared with those of the CDC, Fay-Prince,
counterflow geometry trap (CFG) and HLC. All trials were conducted outdoors
between 06:00 pm-08:00 pm. Female specimens of An. darlingi
were dissected to determine their parity. A total of 8,334 anophelines were
captured, of which 4,945 were identified as An. darlingi . The
best trap configuration was an all-white version, with an upward airflow and no
required light source. This configuration was subsequently named BG-Malaria
(BGM). The BGM captured significantly more anophelines than any of the other
traps tested and was similar to HLC with respect to the number and parity of
anophelines. The BGM trap can be used as an alternative to HLC for collecting
anophelines.
INTRODUCTION: Knowledge concerning the fauna of Culicidae in the Brazilian Amazon States contributes to current understanding of the bionomics of the insects collected and makes it possible to observe changes in the fauna over time. METHODS: The Culicidae were captured with a BG-Sentinel® trap in extra-domiciliary area of two rural regions of Porto Velho in June and July of 2007 and 2008. RESULTS: A total of 10,695 Culicidae was collected, belonging to nine genera: Coquillettidia, Culex, Mansonia, Psorophora, Aedes, Aedeomyia, Anopheles, Uranotaenia and Wyeomyia. CONCLUSIONS: The presence of Mansonia (Mansonia) flaveola was recorded in the State of Rondônia for the first time.
Mosquito colony maintenance in the laboratory is essential for research but presents logistical and ethical problems with the use of live animals for bloodfeeding. The Glytube is an artificial bloodfeeding system for mosquitoes that uses Parafilm-M® membrane and human blood to feed Aedes aegypti. This study evaluated the efficiency of Glytube with different types of membranes and chicken blood to feed Ae. aegypti and Ae. albopictus. We evaluated 2 artificial (thread seal tape [TST], Parafilm-M) and 2 natural membranes (pork, sheep intestine). The results for Ae. aegypti suggest that TST was the best membrane because it presented a high percentage of fed females (63%), a high average number of eggs per female (54.65), and an egg viability rate significantly similar to control (mouse). For Ae. albopictus, there was no significant difference between the membranes and the control; however, the use of TST is suggested due to the low cost and easy manipulation. The treatments that used chicken blood did not present significant differences in the egg viability when compared with the control. The Glytube functionality can be increased by replacing the Parafilm-M membrane by TST and human to chicken blood.
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