SummaryGiven the large applicability in both pharmaceutical and cosmetic field, this work was aimed at analytical (high-performance thin-layer chromatography [HPTLC] method), antioxidant (2,2-diphenyl-1-picrylhydrazyl [DPPH] method), and antimicrobial (diffusion method on Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231) studies on a series of whole vegetal extracts and corresponding aqueous, ethyl acetate and chloroform fractions (selective extracts) prepared from eight plant species growing in Romania. Briefly, it was revealed moderate to certain activity against S. aureus ATCC 6538 and E. coli ATCC 8739 in the case of greater burdock leaves (Arctium lappa), beech leaves (Fagus sylvatica) and great willowherb aerial part (Epilobium hirsutum) whole extracts. Purple loosestrife aerial part (Lythrum salicaria) and sea-buckthorn leaves (Hippophae rhamnoides) whole extracts showed only weak activity against these bacteria, while tarragon aerial part (Artemisia dracunculoides), chokeberries leaves
Abstract:This study aimed to evaluate polyphenols content, antioxidant and antimicrobial activities of ethanol extract from rock rose (Helianthemum nummularium (L.) Mill.). Plant material has been harvested in July from Romanian Carpathian Mountains. The ethanolic extract (70%, v/v) was prepared from the aerial part of rock rose species. High performance thin layer chromatography (HPTLC) analysis of ethanol extract and subsequently hydrolysed sample indicated that quercetin glycosides were the major polyphenolic compounds, and kaempferol derivates, chlorogenic and gallic phenylcarboxylic acids are also being present in the polar extracts. Chemiluminescence assay in luminol/H 2 O 2 system indicated very high antioxidant activity of the ethanolic type of extract (IC 50 = 1.27 μg/mL), while microbiological studies (cylinder method in plates) indicated certain antimicrobial activity (measuring from 12.5 mm to 21.5 mm of the diameter of the inhibition zone) of the propylene glycol (20%, v/v) standardized extract (5 mg gallic acid equivalents/mL sample) against several standard bacterial strains, Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739, Salmonella typhimurium ATCC 14028 and Salmonella enterritidis ATCC 13076, respectively. Therefore, based on the augmented antioxidant effect of the rock rose polar extracts, there can be found numerous applications in the pharmaceutical industry, but also in non-pharmaceutical fields, for example in cosmetic and hygiene products areas, due to certain antimicrobial properties.
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