We studied the expression of MMP-2, MMP-9, and inhibitor TIMP-1 in myocardial autopsy samples from subjects of different age and in cardiomyocyte cultures in the norm and in dilated cardiomyopathy. In autopsy samples of normal myocardium and in cardiomyocyte cultures, expression of molecules involved in extracellular matrix remodeling did not change during aging. In dilation cardiomyopathy, expression of MMP-2, MMP-9, and TIMP-1 and their ratios in autopsy material and in cultures was elevated by 1.5-9 times. Remodeling of extracellular matrix plays an important role in the pathogenesis of dilated cardiomyopathy. MMP-2, MMP-9, and their inhibitor TIMP-1 and the MMP/TIMP ratios can be regarded as promising predictors of dilated cardiomyopathy and used for evaluation of the effectiveness of treatment of this conditions in patients of different ages.
ГБУЗ Архангельской области «Первая городская клиническая больница им. Е. Е. Волосевич», г. Архангельск, Россия 2 ФГБОУ ВО «Северный государственный медицинский университет» МЗ РФ, г. Архангельск, РоссияИшемический инсульт -частая причина инвалидизации и смертности� Стандартной терапией для пациентов, поступивших в течение 4,5 ч от начала симптомов и при отсутствии противопоказаний, является системный тромболизис� В отличие от этого, интервенционная терапия является новым вариантом лечения при окклюзии магистральных артерий головного мозга� Приведено описание клинического случая: 71-летний пациент с инфарктом головного мозга в бассейне правой средней мозговой артерии поступил в срок, но имел противопоказания для системной тромболитической терапии, ему выполнено комбинированное интервенционное лечение с хорошим функциональным восстановлением� Ключевые слова: инфаркт головного мозга, интервенционная терапия, тромболитическая терапия, механическая тромбэктомия
Background: Matrix metalloproteinases (MMP), their TIMP inhibitor, adhesion molecules, sirtuins, and apoptosis markers are among the signaling molecules that may have an important prognostic value in dilated cardiomyopathy. The aim of the study: To study the expression of MMP proteins during myocardial aging in normal conditions and in dilated cardiomyopathy. Materials and methods: The work was performed on autopsy material of the myocardium of people of different ages with dilated cardiopathy and without cardiovascular pathology. The material was taken under sterile conditions of the operating unit. Immediately after taking a sample of tissue, it was placed in a sterile container with saline. The control was the culture of normal human cardiomyocytes (line Girardi Heart, obtained from the cell culture collection of the Institute of Cytology RAS (St. Petersburg). For immunocytochemical studies of cultures and immunohistochemical analysis of autopsy material, the following primary monoclonal antibodies were used: MMP-2 (Dako, USA) and MMP-9 (Dako, USA). The morphometric study of the structures obtained was performed using a computer-aided microscopic image analysis system. Results: It was established that the area changes expression of MMP-2, MMP-9 was not normally observed in the myocardial autopsy material. In DC, the area of MMP-2 expression increased 5.7 times in middle-aged people, 6 times in elderly people and 6.8-fold in elderly patients compared with the corresponding indicator in normal conditions. The expression of MMP-9 in the myocardium in patients with DK of all age groups increased by 4.5 times compared with the corresponding age norm. In normal myocardial cultures with their aging, the expression of MMP-9 did not change significantly. Conclusion: Matrix metalloproteinases are the key markers characterizing the presence of dilated cardiomyopathy in people of different ages in the autopsy and cultural material.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.