We performed chloroplast genome sequencing and comparative analysis of two Rutaceae species, Zanthoxylum schinifolium (Korean pepper tree) and Z. piperitum (Japanese pepper tree), which are medicinal and culinary crops in Asia. We identified more than 837 single nucleotide polymorphisms and 103 insertions/deletions (InDels) based on a comparison of the two chloroplast genomes and developed seven DNA markers derived from five tandem repeats and two InDel variations that discriminated between Korean Zanthoxylum species. Metabolite profile analysis pointed to three metabolic groups, one with Korean Z. piperitum samples, one with Korean Z. schinifolium samples, and the last containing all the tested Chinese Zanthoxylum species samples, which are considered to be Z. bungeanum based on our results. Two markers were capable of distinguishing among these three groups. The chloroplast genome sequences identified in this study represent a valuable genomics resource for exploring diversity in Rutaceae, and the molecular markers will be useful for authenticating dried Zanthoxylum berries in the marketplace.
Recently, safety diagnosis of the existing structures has been emerged as important issue. In particular, systematical and precise safety diagnostics for steel structures for power substation, have been required. Steel structures for power substation are under the periodical impact loads from operations of gas insulated switchgear. These loading condition accelerates damage and aging of structure. The objective of this research is to evaluate damage of structure under periodical impact loads. To evaluate the integrity of structures as organizing mathematical models including the dynamic characteristics of structures, Frequency Domain Decomposition method was choiced and an algorism was proposed. For verifying this methods and algorism, a mathematical model is composed of the development of a variety of reverse analysis and a signal processing technology reflecting physical damage of structures. A series of analysis and test results indicatge that proposed method has a confidence for applying a filed test. Therefore, it is expected to be able to take advantage of system identification to detect damage for the maintenance and management of steel structures under periodical impact loads such as power substation.
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