Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in an ongoing global pandemic crisis, caused by the life-threatening illness coronavirus disease 2019 (COVID-19). Thus, the rapid development of monoclonal antibodies (mAbs) to cope with COVID-19 is urgently necessary. In this study, we used phage display to develop four human mAbs specific to the receptor-binding domain (RBD) of SARS-CoV-2. Our intensive in vitro functional analyses demonstrated that K102.1, an anti-SARS-CoV-2 RBD-specific mAb, exerted potent neutralizing activity against pseudoviral and live viral infection and the interaction between SARS-CoV-2 RBD and human angiotensin-converting enzyme 2. Monotherapy with K102.1 also revealed the therapeutic potential against SARS-CoV-2 infection in vivo. Further, this study developed a sandwich enzyme-linked immunosorbent assay with a non-competing mAb pair, K102.1 and K102.2, that accurately detected the RBDs of SARS-CoV-2 wild-type and variants with high sensitivity in the picomolar range. These findings suggest that the phage-display-based mAb selection from an established antibody library may be an effective strategy for the rapid development of mAbs against the constantly evolving SARS-CoV-2.
Sapovirus (SaV), a virus residing in the intestines, is one of the important causes of gastroenteritis in human beings. Human SaV genomes are classified into various genogroups and genotypes. Whole-genome analysis and phylogenetic analysis of ROK62, the SaV isolated in South Korea, were carried out. The ROK62 genome of 7429 nucleotides contains 3 open-reading frames (ORF). The genotype of ROK62 is SaV GI-1, and 94% of its nucleotide sequence is identical with other SaVs, namely Manchester and Mc114. Recently, SaV infection has been on the rise throughout the world, particularly in countries neighboring South Korea; however, very few academic studies have been done nationally. As the first whole-genome sequence analysis of SaV in South Korea, this research will help provide reference for the detection of recombination, tracking of epidemic spread, and development of diagnosis methods for SaV.
The effect of different plastic deformation methods on the phase composition, lattice defect structure and hardness in 316L stainless steel was studied. The initial coarse-grained γ-austenite was deformed by cold rolling (CR) or high-pressure torsion (HPT). It was found that the two methods yielded very different phase compositions and microstructures. Martensitic phase transformation was not observed during CR with a thickness reduction of 20%. In γ-austenite phase in addition to the high dislocation density (~10 × 1014 m-2) a significant amount of twin-faults was detected due to the low stacking fault energy. On the other hand, γ-austenite was gradually transformed into ε and α’-martensites with transformation sequences γ→ε→α’ during HPT deformation. A large dislocation density (~133 × 1014 m-2) was detected in the main phase (α’-martensite) at the periphery of the disk after 10 turns of HPT. The high defect density is accompanied by a very small grain size of ~45 nm in the HPT-processed sample, resulting in an very large hardness of 6130 MPa.
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