Genotypic diversity among 26 isolates of Bartonella bacilliformis obtained from different areas of Peru, and at different times, was assessed by comparison of DNA sequences derived from 16S-23S ribosomal DNA intergenic spacer regions (ISR) and a citrate synthase gene (gltA) fragment and by amplified fragment length polymorphism (AFLP) analysis. gltA comparison divided the isolates into two groups, whereas ISR comparison revealed six sequences. AFLP analysis using a selective primer delineated five profiles that correlated well with those obtained by sequence comparison. Combination of all three data sets divided the isolates into six genotypes. One of these genotypes was common to isolates collected from a large area in western Peru that corresponded to the region of endemicity for bartonellosis; however, isolates belonging to two other genotypes were also found within this region. Two of these genotypes were found in isolates isolated more than 35 years apart. The remaining three genotypes were each specifically associated with three outbreaks of bartonellosis that have recently occurred in areas where the disease had not previously been recognized. Demonstration of the unique nature of these isolates indicates that the outbreaks with which they were associated did not result from the introduction of disease by individuals who acquired their infection in the recognized region of endemicity. The sources of these outbreaks remain unknown. A consensus approach to bacterial typing using comparative sequence analysis of multiple genetic loci and the pan-genomic sampling of AFLP appears to offer a well-supported assessment of B. bacilliformis diversity, and the genotypic differences identified appear to have epidemiological significance.
Estudio clínico epidemiológico de bartonelosis humana en el valle del Monzón, Huamalíes, Huánuco
Bartonella henselae ha sido recientemente reconocida como agente infeccioso de cuatro síndromes clínicos: angiomatosis bacilar, peliosis bacilar, fiebre y bacteremia, y la enfermedad del arañazo del gato. La bacteria ha sido aislada y completamente caracterizada. La aplicación de nuevas metodologías que aislan, amplifican y analizan el ADN de B. henselae han ayudado a resolver los problemas de identificación y diagnóstico, así como están redefiniendo nuestra comprensión de algunos de los síndromes ocasionados por la bacteria. En los últimos años se ha reconocido que la población en riesgo para las infecciones por B. henselae se ha extendido de los adultos infectados con el virus de la inmunodeficiencia humana a pacientes inmunocomprometidos por trasplante y, luego, a adultos inmunocompetentes y niños. En este artículo se hace una revisión de la microbiología de la bacteria, la epidemiología, la presentación clínica y el tratamiento de los procesos infecciosos ocasionados por B. henselae. Además, se presenta información sobre la respuesta inmunitaria del hospedero y sobre los métodos de diagnóstico e identificación de la bacteria. El propósito de esta revisión es suministrar la información necesaria para estimular la búsqueda de B. henselae en pacientes peruanos.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.