Background: Exposure to heat waves could result in adverse effects on human health, especially in male testicles. PIWI-interacting RNA (piRNA) is a novel type of small non-coding RNA, which can notably impact mRNA turnover and preserve germline maintenance in germline cells. However, piRNA's expression status when adapting to testicular heat stress remains largely unclear.
Objectives:To investigate the function and mechanisms of relevant piRNAs during testicular heat stress.
Materials and methods:In this study, a mouse testicular heat stress model was constructed, and the testes were removed for piRNA-sequencing. Bioinformatics analysis was used to discover the differential expressed piRNAs, piRNA clusters, and enriched pathways. A cell heat stress model was constructed to validate the top five upregulated piRNAs. Proliferation and apoptosis assays were utilized to validate the function of selected piRNA. Bioinformatics prediction, western blotting, and immunohistochemistry were used to illustrate the downstream mechanisms.Results: Through the bioinformatics analysis, we identified the differential expression profile and enriched pathways of piRNAs and piRNA clusters during testicular hyperthermia. Besides, piR-020492 was proved to be upregulated in heat stress mouse testes and a germ cell model. A series of in vitro assays illustrated that an overexpression of piR-020492 could restrain the proliferation and promote the apoptosis of mouse germ cells. Kyoto Encyclopedia of Genes and Genomes analysis of piRNAgenerating genes in the testicular heat stress model and piR-020492 targeting genes showed that the overlap pathways are adenosine monophosphate-activated protein kinase (AMPK) and insulin pathways. Validation experiments demonstrated that Wenbin Chen and Zhang Zhao contribute equally.
School facilities are over stretched due to the student population explosion in Ibadan South-West Local Government Area of Oyo State. The available classrooms are either dilapidated, uncompleted or with dusty floors that show holes of rodents and ant lion larvae. The study was a descriptive survey design of ex-post facto type. The population for the study was all principals and teachers. The Pearson Product Moment Correlation was used to test hypotheses at 0.05 level of significant. However, it was shown that both school facilities were not statistically significant in relating to graduates from upper basic education. Conclusions were made that School facilities are very strong predictors of enrollment into JSS1. There are supply of school facilities for UBE implementation but not adequate. As government strive to provide more found, school authority should put strict measure against students' destructive activities for durability of the school facilities.
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