Shoot proliferation of lingonberry (Vaccinium vitis-idaea ssp. minus) was achieved in vitro from shoot tips excised from 1-year-old stock plants and cultured on a low-salt medium supplemented with 2iP. Rooting was obtained without auxin application by placing microshoots in a peat-vermiculite medium in a high humidity chamber. After 6 weeks, the rooted plantlets were transplanted into a peat-lite mix, subjected to a 2-week low humidity acclimation process and subsequently acclimated in a greenhouse under 50% shade. Within 9 months, these plantlets developed a rhizomatous growth habit, in contrast to the nonrhizomatous habit of conventionally rooted cuttings. Chemical names used: N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP).
Rapid clonal propagation of Alnus glutinosa was achieved in vitro using lateral buds excised from greenhouse grown, juvenile stock plants. Multiple shoot development occurred in 50% of the cultures after the first subculture (7–8 weeks after initial explanting) using a low salt, woody plant medium containing 1 μM 6-benzylaminopurine (BA). Microshoots were removed from pro-liferating tissues and rooted in a conventional potting medium under high humidity prior to establishment in the greenhouse.
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