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The regulator of G-protein signaling (RGS) proteins play an important role in upstream control of heterotrimeric G-protein signaling pathways. In the genome of the human opportunistic pathogenic fungus Aspergillus fumigatus, six RGS protein-encoding genes are present. To characterize the rgsA gene predicted to encode a protein with an RGS domain, we generated an rgsA null mutant and observed the phenotypes of the mutant. The deletion (Δ) of rgsA resulted in increased radial growth and enhanced asexual sporulation in both solid and liquid culture conditions. Accordingly, transcripts levels of the key asexual developmental regulators abaA, brlA, and wetA are elevated in the ΔrgsA mutant. Moreover, ΔrgsA resulted in elevated spore germination rates in the absence of a carbon source. The activity of cAMP-dependent protein kinase A (PKA) and mRNA levels of genes encoding PKA signaling elements are elevated by ΔrgsA. In addition, mRNA levels of genes associated with stress-response signaling increased with the lack of rgsA, and the ΔrgsA spores showed enhanced tolerance against oxidative stressors. Comparative transcriptomic analyses revealed that the ΔrgsA mutant showed higher mRNA levels of gliotoxin (GT) biosynthetic genes. Accordingly, the rgsA null mutant exhibited increased production of GT and elevated virulence in the mouse. Conversely, the majority of genes encoding glucan degrading enzymes were down-regulated by ΔrgsA, and endoglucanase activities were reduced. In summary, RgsA plays multiple roles, governing growth, development, stress responses, virulence, and external polymer degradation—likely by attenuating PKA signaling.

BACKGROUND: The size of 'Wonhwang' Asian pear fruit may affect fruit quality and physiological disorders differentially during distribution. Thus, this study aimed to evaluate the effect of fruit size on fruit physiological attributes and metabolic responses in terms of soluble carbohydrates and free amino acids in different-sized 'Wonhwang' Asian pears during cold storage and shelf life. RESULTS: The rate of weight loss and the severity of fruit shriveling were higher in small fruit than in large fruit, while the severity of decay was lower during shelf life. Lightness and chroma values were lower in the cortex and core tissues of large fruit than small fruit, but hue angle values were higher in large fruit compared to small fruit. Glucose and fructose were higher in large fruit than in small fruit but sucrose and sorbitol were lower during shelf life after cold storage. The levels of most free amino acids were higher in large fruit than in small fruit, and only γ-aminobutyric acid (GABA) level was lower in large fruit. CONCLUSION: These results indicate that fruit physiological and metabolic responses are differentially affected by fruit size during cold storage and shelf life.

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