In the ciliated protozoan Blepharisma, step‐up photophobic response is believed to be mediated by a novel type of photosensory pigment known as “blepharismins” (BL) that are contained in the pigment granules located just beneath the plasma membrane. We examined the ultrastructure of the pigment granules by freeze‐fracture and thin‐section electron microscopy and proposed a schematic diagram showing the granules' three‐dimensional inner membranous structure. Some of the BL are suggested to be associated with 200 kDa membrane protein. High‐pressure liquid chromatography analysis of pigment species associated with 200 kDa protein obtained from blue forms of Blepharisma (oxyblepharisma) revealed that the 200 kDa protein was associated with five types of oxyblepharismin. The fluorescence intensity was increased when the pigments were dissociated from the 200 kDa protein. The result supports the hypothesis that the pigment–200 kDa complex is able to transduce light energy into signals mediating the photobehavior of Blepharisma.
Druck gemacht! Diels‐Alder‐Reaktionen mit Thiophen wurden unter Hochdruck und lösungsmittelfreien Bedingungen durchgeführt. Beispielsweise reagierte Maleinsäureanhydrid (X=O) mit Thiophen bei 0.8 GPa und 100 °C fast quantitativ zum gewünschten exo‐Addukt (siehe Schema). Weitere Reaktionen unter diesen Bedingungen mit Maleinimid‐ (X=NR) und Acryl‐Dienophilen belegen das Potenzial dieser Methode.
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.