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A rapid and internally consistent technique has been developed to measure the volumetric oxygen transfer coefficient, k~a , i n fermentation systems. The method consists of tracing the dissolved 0 2 concentration of the fermentation broth during a short interruption of the aeration. The O2 concentration trace thus obtained can be analyzed to determine the values of k~a . Additional experiments on prolonged 0 2 starvation, carried out to find the limitation of the technique, suggest that 0 2 uptake rate will vary if a prolonged 12-10 min.) 0 2 starvation occurs.

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