ECG was a safe and reliable method for showing HR, and was used to determine the initiation and the effectiveness of resuscitation in the delivery room.
SummaryHaemophagocytic lymphohistiocytosis (HLH) is a life-threatening syndrome of immune dysregulation and is classified as primary or secondary according to the underlying aetiology. The treatment strategies recommended for these two groups differ substantially; however, it is thought to be impossible to predict the underlying causes of HLH using conventional laboratory tests. Recent studies show that serum levels of soluble interleukin-2 receptor (sIL2R) and ferritin are useful for differentiating some forms of HLH. The present study reports that combinations of common laboratory parameters, such as the percentage of total lymphocytes within the peripheral blood leucocyte population, serum levels of lactate dehydrogenase and the sIL2R/ferritin ratio, are useful for identifying patients with familial haemophagocytic lymphohistiocytosis and for differentiating the underlying aetiology of paediatric HLH during the early course of the disease. These findings suggest that the pathogenesis of HLH differs greatly in terms of innate and adaptive immunity depending on the aetiology and may provide a new approach to unravelling the complex pathophysiology underlying this syndrome.
Dried
blood spots (DBS) are widely used for screening biomolecular
profiles, including enzymatic activities. However, detection of minor
proteins in DBS by liquid chromatography–mass spectrometry
(LC-MS/MS) without pre-enrichment remains challenging because of the
coexistence of large quantities of hydrophilic proteins. In this study,
we address this problem by developing a simple method using sodium
carbonate precipitation (SCP). SCP enriches hydrophobic proteins from
DBS, allowing substantial removal of soluble proteins. In combination
with SCP, we used quantitative LC-MS/MS proteome analysis in a data-independent
acquisition mode (DIA) to enhance the sensitivity and quantification
limits of proteome analysis. As a result, identification of 1977 proteins
in DBS is possible, including 585 disease-related proteins listed
in the Online Mendelian Inheritance in Man.
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