Fexofenadine is a non-sedative and selective peripheral H 1 receptor antagonist prescribed for allergic rhinitis and chronic urticaria. This article deals with a simple, feasible, and sensitive isocratic reverse-phase highperformance liquid chromatographic method for the determination of fexofenadine hydrochloride in bulk drug, pharmaceutical dosage forms and in human serum. The chromatography was carried out at 20 ± 2°C using two different chromatographs and five different stationary phases. The isocratic mobile phase was phosphate buffer pH 7.4 and methanol (methanol-phosphate buffer, 35:65, v/v), detection was made at 218 nm and the mobile phase flowed at 1 ml min -1 . Validation parameters included linearity, accuracy, precision, specificity, limit of detection (LOD), limit of quantification (LOQ), and robustness over a linearity range 5-15 lg ml -1 according to the ICH guidelines (r [ 0.9999), the inter-and intra-day precisions were relative standard deviation (RSD) \ 0.8%. The system suitability was scrutinized by capacity factor, tailing factor, and number of theoretical plates (capacity factor [ 2.0, tailing factor B 2.0, and theoretical plates [ 2000). The retention time for five different stationary phases ranged from 3.78 to 4.15 min. The LOD and LOQ for the procedure were executed on samples containing very low concentrations of analytes on two different commercial brands of detectors.
The fresh specimens of Champia compressa Harvey, Gelidium usmanghanii Afaq-Husain and M. Shameel, Gracilaria foliifera (Forsskål) Børgesen and Hypnea musciformis (Wulfen) J. V. Lamouroux, were collected from the coastal areas of Karachi (Pakistan) and their yield of agar was observed. With preextraction technique the percentage of algal yield was found to range from 13 to 28%. High quality gel strength was determined by modulus of elasticity i.e. (550 – 612g/cm2) and 1090 kPa and extraction time was 2 hrs. The structure of agar and agar contents were investigated by FT-IR spectroscopy for the first time from Pakistan. The results revealed some interesting characters (non sulphated β-D galacto pyranose residues, 3,6 anhydro galactose vibration), which were not reported earlier.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.