The present study aimed at evaluating the effect of a phytogenic mixture in the diet on broiler production. A total of 400 day-old unsexed Cobb broiler chicks were randomly allotted to four treatment groups of 10 replications in a randomised design experiment. The phytogenic feed mixture (BMC) contained equal ratios of black cumin, Moringa oleifera and chicory seeds. The treatment groups were as follows: T1 was fed the basal diet, while T2, T3 and T4 were fed the basal diet supplemented with 0.2%, 0.4% or 0.6% of three BMC mixture, respectively. Results showed that increasing the dietary BMC level could be associated with a gradual but significant increase in body weight and improvement in the feed conversion ratio when compared with the control group. Broiler diets enriched with 0.4% to 0.6% of the BMC mixture reduced gut microbial count of coliforms, E. coli and C. perfringens as well as gut pH, compared to the control group. Increasing the dietary BMC mixture level was associated with gradual but significant decrease in serum total cholesterol, low density lipoprotein concentrations and liver enzymes concentrations. However, there was an increase in the high density lipoprotein concentration, and glutathione peroxidase and superoxide dismutase activity in serum. In conclusion, the BMC mixture could be deemed an effective growth promoter, but further research is needed to evaluate it as a viable alternative to antibiotics.

AbstrtactBackground: This study was aimed to find out the central and peripheral analgesic activity of hydro methanolic extract of aerial parts of Onosma bracteatum. Material and methods: The central and peripheral analgesic activity is evaluated by tail flick test and acetic acid induced writhing test at the doses of 50, 100, 250 and 500mg/kg body weight respectively in animal models. Results: The results obtained from Tail flick test revealed that O. bracteatum possesses potent analgesic effects by inducing significant increase in latency period in dose dependent manner at all doses at 1, 2 and 3 hours post feeding respectively. The maximum effect was observed at a dose of 500mg/kg i.e. 258.9% (p<0.05) at 3hrs post feeding. Diclofenac sodium (5mg/kg body weight) run as standard also increased the latency period continuously and highest activity was noted at 3hr i.e. 284.5% (p<0.05). Acetic acid induced writhing test also showed significant activity in a similar manner by O. bracteatum i.e 54% (p<0.05) at 500mg/kg while standard drug Diclofenic sodium (5mg/kg body weight) showed 45.9% (p<0.05) activity. Conclusion: It is concluded that O. bracteatum possesses significant central and peripheral analgesic activity in animal model.

Background & Objective: The aim of present study was to evaluate herbal analgesic cream containing Nigella sativa oil as an active ingredient, standardization of Nigella sativa oil and evaluation of irritation/sensitization potential of formulation. Material & Method: For this, HPLC analysis of oil, skin irritation test on rabbits and patch testing on human skin was conducted. HPLC analysis using C-18 column, using an isocratic mobile phase of water: methanol (10:90) at flow rate of 1 ml/inactive ingredient: thymoquinone purified from the oil was found to be 1.42g (28.4 %). Safety assessment of analgesic cream was based on Primary Dermal Irritation Index (PDII) by Draize method. Result: According to Draize standard scoring system of reactions PDII was found 0.04 which comes under the category of non irritant. Patch testing on human volunteers revealed that none of volunteers showed any sign of skin reactions. Bangladesh Journal of Medical Science Vol.19(1) 2020 p.163-168

Objective: To investigate and determine the antidiarrhoeal and spasmolytic potential of fruit of Aegle marmelos is due to CCBs compounds and not tannic acid. Materials and methods: The extract of ripe and dry fruit of A. marmelos (Am.Cr) was prepared in methanol: water (70:30). The antidiarrhoeal activities of the extract, loperamide and tannic acid were studied in vivo; in castor oil induced diarrhoeal model in mice whereas spasmolytic effect was studied in vitro; in isolated mice ileum. Calcium channel blocking (CCB) activity was investigated after preincubation of mice ileum by Am. Cr or loperamide and subsequent adding of K + 80 mM. Results: Am. Cr inhibited castor oil-induced diarrhoea: onset of diarrhoea, total no. of faeces, and total no. of wet faeces dose-dependently, the dose of 800 mg/mL was found statistically significant, total weight of feces and total weight of wet faeces were also inhibited. Concentration-dependent inhibition of spontaneous contraction and contractile effect of K + 80 mM after pre-incubation by Am. Cr were confirmed in isolated mice ileum. The results of Am. Cr are comparable with Loperamide. Tannic acid produced neither antidiarrhoeal effect nor exhibited CCB activity, however relaxant effect was observed in isolated mice ileum. Conclusion: The antidiarrhoeal and spasmolytic effects of Am.Cr may be mediated through compounds which posses CCB effect. Tannic acid exhibited relaxant effect only which is not sufficient evidence scientifically to classify as antidiarrhoeal.

Objective: Phoenix dactylifera fruit widely used in Muslim countries.They have religious attachment to it and also it had many health benefits. It is essential to investigate nutritional values of Phoenix dactylifera fruit in dried powder form and to find out any toxicological changes during processing of this dried powder drink.Method: Dried date powder was prepared from fresh fruit by drying it and its nutritional value and toxicological studies were carried out. The acute oral toxicity study of date powder was conducted on wistar strain rats by oral route in a dose of 0.5g/kg body weight and 0.1g/kg body weight.Result: The results of biochemical analysis of dehydrated date powder exhibited that it possesses high energy value of 311 Kcal, carbohydrate 70.5%, dietary fiber 7.3%, fat content 2.1%, protein 2.6%, and mineral content 2.2%. Whereas, total sugar was found to be as 63.2% and moisture content 2.3%. The test drink showed no sign of toxicity or death during the whole observation period (fourteen days) in rats. No group of animal showed any unusual change in behavior or in locomotors activity. The macroscopic studies of vital organs i.e. heart, liver, spleen, lungs and kidneys exhibited that they were normal.Conclusion: As a result of these studies it can be concluded that from processing of fresh fruit to dried powder along with its nutritional and toxicological studies no hazardous material like toxins were produced. So it can be used safely.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.263-269