The present study is concerned with the optimization of growth conditions for Azotobacter species. Five isolates were isolated from soil and Azotobacter IIB-3 found to be the best; along with strain of Azotobacter vinelandii NRRL-14641. The medium M2 gave maximum growth (0.65 and 0.75 mg/ml) of Azotobacter vinelandii and Azotobacter IIB-3. Optimum temperature, pH and incubation period for growth of Azotobacter were 30ºC, 8.0 and 48hrs, respectively. The growth of Azotobacter was also studied with the supplementation of growth medium with different carbon and nitrogen sources. 2.5% mannitol (as C-source) and 2% (NH 4) 2 SO 4 (as N-source) were found best for both Azotobacter vinelandii (1.16mg/ ml) and Azotobacter IIB-3 (1.24mg/ml). 24 hrs old inoculum at a level of 1% was found best for the growth both Azotobacter vinelandii and Azotobacter IIB-3. The effect of Azotobacter biofertilizer was studied on maize plants in pot experiment and it was found that plants inoculated with Azotobacter gave better growth as compared to control plants.
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