In the present study, fatty acid composition in mature seeds of the Vicia L. taxa were determined by using gas chromatography. Present results indicate that the seed oils of studied Lathyrus taxa have palmitic acid (C16:0) and stearic acid (C18:0) as major saturated fatty acids. Moreover, the present data showe that oleic acid (C18:1), linoleic acid (C18:2 n 6) and α-linolenic acid (C18:3 n 3) were major unsaturated fatty acids in studied taxa. The highest oleic acid (C18:1 n 9) content was found in V. crocea (Desf.) B. Fedstch. (26.5%) but it was found at the lowest level in V. mollis (7.8%). In addition, present study determine that investigated Vicia species have linoleic acid (46.4-79.1%) and linolenic acid (3.8-21.3%) contents.
Abstract.-In the present study, levels of vitamin and fatty acid composition in mature seeds of some Lathyrus L. taxa [L. roseus Stev., L. tuberosus L.,var. affinis (Guss.) Arcang.] were determined by using HPLC (high-performance liquid chromatography) and VPC (vapour gas chromatography). Palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1 n-9), linoleic acid (C18:2 n-6) and α-linolenic acid (C18:3 n-3) were found as major fatty acid components in all studied eleven taxa. In addition, δ-tocopherol, α-tocopherol, α-tocopherol acetate and vitamin D3 were found highest level and retinol, retinol acetate, vitamin D2 and vitamin C content (apart from L. belinensis and L. hirsutus) were found lowest ratio in the examined Lathyrus taxa.
Key words : Lathyrus -fatty acids -vitamins -HPLC -VPC.
Résumé.-Dans la présente étude, les niveaux en vitamines et en acides gras des graines adultes de Lathyrusvar. affinis (Guss.) Arcang.] ont été déterminés par chromatographie (CLHP et CG). Les acides palmitique (C16:0), stéarique (C18:0), oléique (C18:1 n-9), linoléique (C18:2 n-6) et α-linolénique (C18:3 n-3) ont été observés comme constituants des onze taxons étudiés. En plus, les δ-tocopherol, α-tocophérol, acétate de l'α-tocophérol et la vitamine D3 ont été trouvés en plus grande quantité, les rétinol, acétate de rétinol, vitamine D2 et vitamine C le l'ont été en quantité faible (sauf L. belinensis et L. hirsutus) pour les Lathyrus examinés.
A new 1H-pyrazole-3-carboxylic acid 2, along with hydrazono-pyridazinone 3, a by-product, and its derivatives 4-7 were synthesized and the structures confirmed by infrared (IR) and 1 H and 13 C nuclear magnetic resonance (NMR) data. These new compounds were evaluated for their antibacterial activities against Gram-positive and Gram-negative bacteria using the tube dilution method. The minimal inhibitory concentrations (MICs) experiments revealed that most compounds exerted inhibitor effects against Klebsiella pneumonia, Escherichia coli, Bacillus subtilus, and Xanthomonas compestris test microorganisms. Moreover, the results showed that the pyrazolo[3,4-d]pyridazine compounds were the best compounds of the series, exhibiting antibacterial activity against both Gram-positive and Gram-negative bacteria.
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