Nitrate can be a source of NPN for microbial growth at the same time that it reduces ruminal methane production. The objective of this study was to evaluate the effects of 2 encapsulated nitrate products used as urea replacers on blood and rumen constituents, methane emission, and growth performance of lambs. Eighteen Santa Inês male lambs (27 ± 4.9 kg) were individually allotted to indoor pens and assigned to a randomized complete block design with 6 blocks and 3 dietary treatments: control (CTL) = 1.5% urea, ENP = 4.51% encapsulated nitrate product (60.83% NO3(-) in the product DM), and ENP+CNSL = 4.51% ENP containing cashew nut shell liquid (60.83% NO3(-) and 2.96% cashew nut shell liquid [CNSL] in the product DM). Diets were isonitrogenous with 60:40 concentrate:forage (Tifton 85 hay) ratio. The experiment lasted for 92 d and consisted of 28 d for adaptation (a weekly 33% stepwise replacement of CTL concentrate by nitrate-containing concentrates) and 64 d for data collection. The ENP and ENP+CNSL showed greater (P < 0.05) red blood cell counts than CTL. Blood methemoglobin (MetHb) did not differ (P > 0.05) among treatments, with mean values within normal range and remaining below 1.1% of total hemoglobin. There was an increase (P < 0.05) in total short-chain fatty acids concentration at 3 h postfeeding for ENP, with an additional increase (P < 0.05) observed for ENP+CNSL. No treatment effects (P > 0.05) were observed on acetate to propionate ratio. Methane production (L/kg DMI) was reduced (P < 0.05) with nitrate inclusion, recording 28.6, 19.1, and 19.5 L/kg DMI for CTL, ENP, and ENP+CNSL, respectively. Addition of CNSL did not result (P > 0.05) in further reduction of methane production when compared with ENP. Final BW, DMI, ADG, and feed efficiency were similar (P > 0.05) among treatments. Values for DMI were 1.11, 1.03, and 1.04 kg/d and for ADG were 174, 154, and 158 g for CTL, ENP, and ENP+CNSL, respectively. In conclusion, encapsulated nitrate products showed no risks of toxicity based on MetHb formation. The products persistently reduced methane production without affecting performance. Inclusion of cashew nut shell liquid in the product formulation had no additional benefits on methane mitigation.

The objective of this study was to evaluate the relationships between chewing behavior, digestibility, and digesta passage kinetics in steers fed oat hay at restricted and ad libitum intakes. Four Hereford steers, with an initial average BW of 136 kg, were used in an experiment conducted as a balanced 4 × 4 Latin square with 4 treatments (levels of intake) and 4 periods. Animals were fed lopsided oat hay (Avena strigosa Schreb.) at 4 levels of intake (as a percentage of BW): 1.5, 2.0, 2.5, and ad libitum. Digestibility, chewing behavior, and digesta passage kinetic measurements were recorded during the experimental period. Chewing rates during eating and ruminating [(chews•min(-1))/g of DMI•kg(-1) of BW•d(-1)] decreased (P = 0.018 and P = 0.032, respectively) with increased DMI (g•kg(-1) of BW•d(-1)), whereas total chewing and total time spent on each chewing activity increased. Calculated total energy expended by the chewing activity was 4.2, 4.4, 5.2, and 5.3% of ME intake for DMI of 1.5, 2.0, and 2.5% of BW and ad libitum, respectively, indicating that adjustments in animal chewing behavior may be a mechanism of reducing energy expenditure when forages are fed at restricted intake. Hay digestibility decreased (P < 0.001) with increased DMI (r = -0.865). Digesta mean retention time (h) was strongly correlated with DMI (r = -0.868) and OM digestibility (r = 0.844). At reduced intake, hay digestibility was enhanced (P < 0.001) by extending digesta retention time and by increasing chewing efficiency, highlighting the relationship between chewing behavior and the digestive process. Fractional outflow rate of particulate matter from the reticulorumen (k(1)) was positively correlated with total chews, emphasizing that the decrease in particle size caused by chewing facilitates particle flow through the digestive tract. Increased hay intake also increased (P < 0.001) k(1), whereas passage rate of the liquid phase, transit time, and rumen fill were not affected (P > 0.05). The latter was correlated with rumen volume (r = 0.803). In conclusion, the results of this study indicate that animals fed at restricted intake increased chewing rate when eating and ruminating, which, along with a longer digesta retention time, contributed to enhance feed digestibility.

RESUMO -Objetivou-se com este trabalho avaliar o efeito do processo de digestão de bovinos sobre o poder germinativo de sementes de capim-annoni-2 (Eragrostis plana Ness) recuperadas nas fezes. As sementes (6 g, 27.273 sementes) foram colocadas no saco ventral do rúmem de oito novilhos fistulados, mantidos em gaiolas para ensaios metabólicos. Quarenta e três por cento das sementes fornecidas aos animais foram recuperadas nas fezes: aquelas recuperadas nos três primeiros dias corresponderam a 97,2% da quantidade de sementes fornecidas. Somente 7,2% das sementes recuperadas, equivalente a 3,1% da quantidade de sementes fornecidas, apresentaram viabilidade. O tempo de permanência no trato digestivo foi a principal causa do desaparecimento das sementes consumidas e da perda de seu poder germinativo. A ausência de sementes de capim-annoni-2 nas fezes ocorre a partir do oitavo dia após a ingestão, assim, bovinos devem permanecer em quarentena por sete dias em área livre de capim-annoni-2 para limpeza do trato digestivo.Palavras-chave: campo natural, dispersão de invasoras, ingestão de sementes, invasão biológica, transporte de sementes Germination of capim-annoni-2 (Eragrostis plana Ness) seeds recovered in bovine fecesABSTRACT -This work was carried out with the objective of evaluating the effect of the bovine digestion process on the germination capacity of the capim-annoni-2 (Eragrostis plana Ness) seeds recovered in feces. The seeds (6 g, 27,273 seeds) were placed in a ruminal ventral sack of eight fistulated steers kept in pen trial. Forthy-three percent of the offered seeds were recovered in the feces. Seeds recovered in the first three days corresponded to 97.2% of the offered seeds. Only 7.2% of the recovered seeds germinated and represented 3.1% of the offered seeds. The permanence time in the digestive trait was the main cause for the disappearance of consumed seeds and for the loss in their germination capacity. The absence of capim-annoni-2 seeds in the feces occurs in the eighth day after ingestion, then, bovines should be kept in quarantine for seven days in an area free of capim-annoni-2 seeds to clean their digestive trait.

idade de 18 meses, em delineamento completamente casualizado. A pastagem apresentou valores médios de 6,8% de proteína bruta, 73,3% de fibra em detergente neutro, e 42,5% de digestibilidade in vitro da matéria orgânica, sem que fossem detectadas diferenças significativas entre tratamentos. O ganho médio diário (0,287 kg) dos animais suplementados com o sal proteinado com amiréia e levedura, foi superior ao apresentado pelos animais que consumiram sal mineralizado (0,019 kg) mas não houve diferenças entre uréia (0,159 kg) e amiréia (0,124 kg). O consumo diário dos suplementos proteinados (0,400 kg) foi superior ao consumo do suplemento mineral (0,038 kg). A adição de levedura ativa, ao sal proteinado formulado com amiréia, melhora o desempenho de novilhos em pastagem nativa diferida.Termos para indexação: suplementação protéica, uréia, amiréia, levedura, campo nativo. Performance of steers supplemented with protein salts on native pasturesAbstract -The objective of this work was to evaluate the effect of protein salts supplementation on performance of beef steers grazing differed native pasture in Rio Grande do Sul State, Brazil. Protein salts were supplemented with: urea, starea, starea plus yeast, and mineral salt. The experiment was conducted during 118 days, and utilized 8 paddocks with 7.5 ha each one, with 8 steers averaging 264 kg and 18 months old, in a completely randomized design. Average composition of pasture was 6.8% of crude protein, 73.3% of neutral detergent fiber, and 42.5% in vitro organic matter digestibility; significative differences weren't detected among treatments. Average daily weight gain of animals fed protein salt with starea plus yeast (0.287 kg), was higher than the weight gain of animals fed mineral salt (0.019 kg); differences weren't detected among urea (0.159 kg) and starea (0.124 kg). Daily intake of protein supplements (0.400 kg) was higher than that of mineral supplement (0.038 kg). Addition of live yeast to the protein salt formulated with starea resulted in better performance of steers grazing differed native pasture.