Abyssomicin C:A Polycyclic Antibiotic from a Marine Verrucosispora Strain as an Inhibitor of the p-Aminobenzoic Acid/Tetrahydrofolate Biosynthesis Pathway. -The structure of the new polycyclic polyketide-type antibiotic abyssomicin (I) is elucidated. -(BISTER, B.; BISCHOFF, D.; STROEBELE, M.; RIEDLINGER, J.; REICKE, A.; WOLTER, F.; BULL, A. T.; ZAEHNER, H.; FIEDLER, H.-P.; SUESSMUTH*, R. D.; Angew.
The screening for growth inhibitors against Bacillus subtilis revealed a peptide antibiotic produced by Streptomyces griseoflavus TU 4072. Very high yields of the antibiotic named aborycin were obtained by cultivation in a medium containing soybean meal and sucrose. Isolation from the mycelium was carried out by a combination of extraction and gel chromatography. Preparative HPLC yielded the uniform antibiotic. The structure elucidation was based on GC amino acid analysis on a chiral phase, automated Edman degradation,The Streptomyces griseoflavus strain TU 4072 was isolated from a soil sample collected in Alice Springs, Australia. Due to this origin of the producing strain the isolated antibiotic was named aborycin. Cultivation using soybean meal as nitrogen source and sucrose as carbon source gave high yields of aborycin. For the isolation of the antibiotic the aqueous methanolic extract of the mycelium was first extracted with ethyl acetate and then with l-butanol. Gel chromatography of the 1-butanol extract followed by preparative HPLC gave 271 mg of pure aborycin out of 5 liters of culture broth. After treatment with Ehrlich's reagent the antibiotic gave a blue spot on TLC plates (Rf = 0.47, 1-butanoYethanoVwater, 6:2:2). The Rf value was similar to the one of tryptophan. Aborycin inhibited the growth of Bacillus subtilis ATCC 6633 at a minimum inhibition concentration (MIC) of 20 pg/ml, Bacillus brevis ATCC 9999 (MIC 25 pglml), Staphylococcus aureus ETH 2070 (MIC 15 pglml), Pseudomonas saccharophila ATCC 15946 (MIC 15 pg/ml), and of Streptomyces viridochromogenes TU 57 (MIC 2 pglml) but was inactive against Escherichia coli K12, Salmonella typhimurium TU 5108, Mucor hiemalis TU 179/180, Mucor miehi TU 284, and Yarrowia lipolytica TU 5054.
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