This study evaluated, by morphologic and morphometric analyses, the cleaning of apical third of root canals instrumented with nickel-titanium rotary files using different irrigating solutions. Twenty-seven single-rooted mandibular premolars were assigned to three groups (n=9), according to the irrigating solution used: Group I, distilled and deionized water; Group II, 1% NaOCl; and Group III, 3.3% Ricinus communis detergent. Biomechanical preparation was performed with Protaper Plusâ nickel-titanium files as follows: S1, SX and S2 at the cervical and middle thirds, and 25/02, 25/04, 25/06, 30/02, 30/04 and 30/06 to complete the instrumentation, operating at 1 mm from the root apex. Irrigation was done at each file change with 2 mL of irrigating solution, totalizing 20 mL for each tooth. After biomechanical preparation, the apical thirds were serially sectioned and histologically processed. The cross-sections were examined by an optical microscope (X40) connected to a computer. The images were captured and analyzed using a computer software and submitted to morphometric analysis with aid of a grid. The percentage of debris remaining at the apical third was calculated. Data were submitted to statistical analysis by ANOVA and Tukey test. The results showed no statistically significant difference (p>0.01) between the groups irrigated with 1% NaOCl and 3.3% Ricinus communis detergent, which presented the lowest percentage of debris at the apical third, 8.49% and 10.11%, respectively. The group irrigated with distilled and deionized water had the highest percentage of debris (15.58%) and was statistically different from the other groups (p<0.01). It may be concluded that 3.3% Ricinus communis detergent and 1% NaOCl had similar cleaning effectiveness on removal of debris from root canals.
In vitro tumor growth inhibition by the ethanol extract of Miconia fallax DC. (Melastomataceae) was evaluated in culture media containing HeLa cells at the following concentrations: 5, 15, 25, 35, and 45 µg/mL. Bioassay-guided fractionation of this extract furnished a mixture of ursolic and oleanolic acids. Both the ethanol extract and the mixture of the triterpenoid acids produced dose-dependent tumor growth inhibition. This antitumor activity was more pronounced when a mixture of the triterpene acids at a concentration of 45 µg/mL was added to a HeLa cell culture medium.
ResumoNeste presente estudo foram comparadas duas composições de pastas medicamentosas para utilização intracanal: pasta de hidróxido de cálcio e óleo de Ricinus communis e pasta de hidróxido de cálcio e polietilenoglicol. Tubos de polietileno foram implantados no tecido subcutâneo de 9 ratos, sendo 2escapulares e 2 pélvicos, totalizando 4 tubos por animal, cada par com uma das pastas. O grupo controle foi definido pela lateral do tubo. Após 7, 21 e 42 dias os animais foram sacrificados e os tecidos processados para obtenção dos cortes histológicos e posterior análise histopatológica. Os resultados foram classificados quanto à severidade da reação inflamatória e quantificados em relação aos eventos presentes nas áreas pré-determinadas. Observou-se à análise morfológica, que ambas as pastas apresentaram índice de inflamação intenso aos 7 dias - pasta A (29,7) e pasta B (39,3), persistindo até aos 21 dias (pasta A – 27,1; pasta B – 27,9). Aos 42 dias a reação tecidual foi moderada (pasta A – 12,4; pasta B – 13,0). Comparando-se ao grupo controle, percebeu-se que este apresentava reação tecidual indo do moderado (14,2 aos 7 dias e 10,0 aos 21 dias) ao discreto (7,8 aos 42 dias). Quando submetidos à análise estatística (ANOVA – Tukey – p< .01) esses valores apresentaram diferença estatisticamente significante para todas as pastas e grupo controle. Concluiu-se que ambas as pastas apresentaram-se como irritantes ao tecido conjuntivo, permitindo colagenização progressiva da cápsula junto à abertura tubular. Porém, a pasta com óleo de Ricinus communis (Pasta A) apresentou potencial irritativo estatisticamente menor que a Pasta B.
This study allowed the characterization of the tambaqui Colossoma macropomum testes structural organization, emphasizing Sertoli and interstitial cells and analyzing morphometrically the Sertoli cell nucleus diameter and the interstitial tissue area during the reproductive cycle. Fragments of tambaqui testes were collected in the following reproductive cycle stages: immature, resting, maturation I and II, mature, and regression, and were histologically processed. The Sertoli cells were found at the periphery of the cysts of germinative lineage cells and the nuclei were shown to be smaller as these cells developed. The interstitial cells were better observed between the seminiferous lobules next to vessels in the interstitial tissue of maturing testes.
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