Molecularly imprinted microspheres for recognition of chlorpyrifos were prepared by thermal initiation and precipitation polymerization, and were characterized using scanning electron microscope and infrared spectroscopy. Binding characteristics of the microspheres were also investigated. Sensitive film electrode of chlorpyrifos was prepared using the microspheres above on the surface of gold electrode by spin coating. Electrochemical sensor was constructed with the electrode prepared above as recognition element, and the response characteristics of the sensor to chlorpyrifos in water were investigated based on constant-current potentiometry. It was shown that a reasonable linear response curve between potential and concentration was obtained from 1.0×10-12mol/L to 2.0×10-8mol/L, with a detection limit of 1.0×10-13mol/L. The suitable pH was 2.2~3.4,and response time was 10 min. The imprinted electrode showed relatively high selectivity to chlorpyrifos and was applied to the analysis of chlorpyrifos in the simulated river samples with recovery rates ranging from 89% to 105%.
The present study was designed to elucidate the Catalase (CAT) activity and malondialdehyde (MDA) contents in gills and muscle tissues of Zebrafish in exposure to different concentrations of deicing salt. According to the value of 96 h LC50, five different concentration treatments (4.30, 5.38, 6.73, 8.41 and 10.51 g/L) of deicing salt were set up with no application of deicing salt (0 g/L) as control treatment, and the activity of CAT and MDA in the gills and muscle tissues of Zebrafish were measured at 1d, 4d, and 7 d exposure. The results showed that the 96 h LC50 value of deicing salt for Zebrafish was 13.49 g/L and the safe concentration was 4.26 g/L. The CAT activity in muscle tissues of Zebrafish was activated significantly (P<0.01) at 1d, and was inhibited significantly with the concentration of 10.51 g/L (P<0.05) by comparing with the control treatment during the whole experimental time. However, the CAT activity in gills was activated significantly (P<0.01) with the concentration of 5.38 g/L, and inhibited significantly (P<0.05) with the concentration of 10.51 g/L. The MDA contents in gills of Zebrafish enhanced gradually with the deicing salt concentration increased. In muscle tissues of Zebrafish, MDA contents decreased gradually during the whole experimental time.
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