NAC proteins play an essential role in the growth and development of litchi, especially during reproductive development. However, a comprehensive analysis of the litchi NAC gene family is currently absent. Based on information from the litchi genome, we found that the 112 NAC genes of litchi show an uneven distribution on the chromosomes. Phylogenetic and conserved structural domain analyses indicated that different types of variability were exhibited in the family of litchi NACs (LcNACs). Gene covariance analysis showed that the LcNACs showed better similarity in the same genus than with Arabidopsis. We further investigated the differential expression patterns of LcNACs in buds and rudimentary leaves of litchi. qRT-PCR results implied that they were involved in the process. Profiling of LcNAC promoter elements in litchi showed that they were extensively involved in light response, phytohormone regulation, abiotic stress response, and plant growth and development processes. This study provides new insights into the identification, structural characterization, tissue-specific expression analysis, and promoter response elements of LcNACs. It reveals the characteristics of the LcNACs and lays the foundation for the subsequent understanding of its biological functions and molecular regulatory mechanisms.
Influence of Differentially Expressed Genes on Thyroid CancerThyroid carcinoma poses a heavy disease burden on the global health. The potential influence of some differentially expressed genes on the progression of thyroid carcinoma was still incomplete. In this study, we integrated transcriptome data with clinical data to investigate the relationship between them in thyroid cancer patients. First, the gene expression profile (GSE129562) from Gene Expression Omnibus was used to identify differentially expressed genes. Secondly, gene ontology function and Kyoto encyclopedia of genes and genomes pathway enrichment analyses of the differentially expressed genes were performed. Thirdly, protein-protein interactions of the differentially expressed genes were constructed. Between T1aN1b or T3N1b thyroid carcinoma and its paired thyroid normal tissue, a total of 729 differentially expressed genes were identified through our analysis, of which 405 genes were up-regulated and 324 genes were down-regulated. Of those differentially expressed genes, 138 differentially expressed genes were identified as immune-related genes. Their functions can be classified as antigen processing and presentation, antimicrobials, B cell receptor signaling pathway, chemokine receptors and so on. Gene ontology enrichment analysis illustrated that these differentially expressed genes were mostly enriched into extracellular matrix structural constituent, integrin binding and so on. Kyoto encyclopedia of genes and genomes enrichment analysis illustrated that these differentially expressed genes were mostly enriched into extracellular matrix-receptor interaction, focal adhesion and so on. The top 4 differentially expressed genes with the highest degrees in the protein-protein network are syndecan 1, tyrosine-protein kinase Fyn, decorin and thrombospondin-1. The mutations in the gene solute carrier family 34 member 2 and tenascin C are arguably the most common. Our results demonstrate the potential influence of some differentially expressed genes on the progression of thyroid cancer, provides a comprehensive bioinformatics analysis of the pathogenesis, which may contribute to future investigation into the molecular mechanisms and biomarkers.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.