Precise calculations of growing degree days (GDD) are an important component in crop simulation models and managerial decisions. Traditional methods for calculating GDD assume linear developmental responses to temperature and cannot precisely account for the delay in growth or development at temperatures above the optimal temperature (Topt). A new nonlinear method for calculating GDD was developed. Variations in the prediction of the dates since sowing to various developmental stages and performance measures for describing the accumulation of dry matter by GDD for two widely planted crops (corn and wheat) were used to evaluate the new method in comparison with the traditional methods. The new method predicted the dates of the developmental stages more precisely (date variations reduced by 1 d), and the errors for the predictions of the accumulation of dry matter for winter wheat and corn were smaller. The method was most promising for spring wheat. The new method was more stable and more precise than traditional methods, especially when Topt was lower than the maximum air temperature.
A microlens fluorescence imaging system and a panoramic image reconstruction algorithm for biological cell fluorescence detection are presented in this paper. Biological cell samples are labeled with fluorescent dyes and made into sections in a short time. The imaging system in this paper can detect the fluorescence of the samples. By modeling the optical path of the microlens imaging system and the two-dimensional mobile scanning imaging mode, the better fluorescence detection effect is obtained with the panoramic image reconstruction algorithm. The experimental results show that the microlens fluorescence imaging system can successfully collect the fluorescence information, and can reach the resolution of 2.68μm in the image sensor frame area. At the same time, the field of view area of the imaging system can reach at least 9 times or more of the sensor frame area after the reconstruction algorithm, which realizes the rapid and convenient cell fluorescence detection with a larger field of view.
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