The cryopreservation of testicular tissue is presented as the only alternative for the preservation of genetic material from prepubertal animals. However, this biotechnology is still being tested. The objective of this study was to evaluate the effect of different associations of cryoprotectants and the potential of cell proliferation after vitrification of testicular tissue of prepubertal cats. Five testicular pairs from five prepubertal cats were used, and each pair was divided into four fragments. Of these, one fragment composed of the control group (CG) and the rest were distributed in experimental groups according to the associations of cryoprotectants to be tested (dimethyl sulphoxide (DMSO)/glycerol (GLY); ethylene glycol (EG)/GLY) or DMSO/EG) in a final cryoprotectant concentration of 5.6 m. The fragments were submitted to vitrification, and after one week, fragments were heated and processed for histomorphological evaluation and quantification of nucleolar organizer regions (NORs). DMSO/GLY did not differ from CG and was superior to the other vitrified groups, as to cell separation and degree of shrinkage of the basal membrane. Concerning cell differentiation, visibility of the nucleus and nuclear condensation, all the vitrified groups were inferior to CG; however, DMSO/EG was inferior to DMSO/GLY and EG/GLY, which did not differ among themselves. CG was superior to all groups in quantification of NORs. DMSO/EG was inferior to all others, and there was no difference between DMSO/GLY and EG/GLY. The association DMSO/GLY presented the best preservation of tissue integrity and potential of cell proliferation after vitrification of the testicular tissue of prepubertal cats.
(-)-α-Bisabolol has a nephroprotective effect in kidney I/R, with antioxidant effect. Moreover, this result seems to be associated to a direct protective effect on tubular epithelia.
A histoplasmose é uma doença fúngica sistêmica, causada pelo fungo Histoplasma capsulatum, este caracteriza-se por ser dimórfico e saprófita. Esta patologia pode ter caráter clínico agudo ou crônico e resultar em infecção subclínica, pulmonar ou disseminada, caracteriza-se por ser a segunda micose de maior ocorrência nos gatos e nesses apresenta baixa morbidade e altas taxas de mortalidade. O diagnóstico pode ser obtido pela cultura fúngica e o tratamento é realizado principalmente a base de itraconazol. Objetivando relatar um caso clínico de histoplasmose felina realizou-se o presente trabalho. Foi atendido um gato sem raça definida, fêmea, de quatro anos de idade, com queixa principal de aumento de volume nasal. Foram solicitados inicialmente exames hematológicos, bioquimicos, citológico da nodulação no plano nasal, teste imunocromatográfico para imunodeficiencia viral felina e leucemia viral felina, e sete dias após, foi solicitada a cultura fúngica. Nos exames hematológicos observou-se trombocitopenia, agregados plaquetários, leucocitose por neutrofilia e hipoalbuminemia. O exame imunocromatográfico para ambas as retroviroses foi negativo. No exame citopatológico foram encontradas estruturas leveduriformes sugestivas de Criptococcus sp.,todavia, na cultura fúngica essas estruturas foram compatíveis com Histoplasma capsulatum. Baseado no resultado do exame citológico, foi prescrito para a paciente itraconazol 10 mg/kg duas vezes ao dia durante 60 dias, este deveria ser iniciado após a realização da cultura fúngica. O animal apresentou melhora significativa a partir de 7 dias do uso do medicamento, com regressão total da nodulação após 26 dias de tratamento. Após os 2 meses de tratamento, houve suspensão da medicação por 19 dias por decisão da tutora e retorno da administração por mais 30 dias no intuito de evitar a remissão da patologia. Com isso pode-se concluir que a histoplasmose nasal é uma afecção fúngica presente na rotina clínica de felinos, exigindo assim do médico veterinário atenção para a sintomatologia e métodos de diagnósticos complementares, bem como, a realização do tratamento e prognóstico de forma correta.
Chronic kidney disease (CKD) is a relevant disease in feline clinic. The tubulointerstitial damage, with collagen deposition and fibrosis, is an important result of this process. The aim of this study was to quantify and correlate the deposition of collagen and severity of interstitial fibrosis (IF) in the kidney from cats in different stages of CKD. Kidney fragments from 10 adult cats with CKD were analyzed and stained by Masson's trichrome (MT) and Picrosirius red (PSR) for circular polarized microscopy. Random quantitative analysis was performed on MT sections to classify the degree of IF, per field area, with and without circular polarization. Statistics correlations were performed by Spearman's (ρ; p < .05). There was a significant correlation of IF quantification with the area of interstitial collagen deposition by polarized PSR (PSRp) (r = .7939, p = .0098) and nonpolarized PSR (PSRn) (r = .7781, p = .0080). There was a positive correlation of serum creatinine (sCr) at different stages of CKD with PSRp (r = .7939, p = .0098), PSRn (r = .8667, p = .0027) and MT (r = .7818, p = .0117). Correlations between the percentage of quantified area was also positive from PSRp to PSRn (r = .9030, p = .0009) and PSRp to MT (r = .7939, p = .0098). The PSRN was also correlated with MT (r = .9273, p = .0001). The correlation with IF and sCr follows the disease evolution and the quantification of collagen by PSR is an excellent tool for analyzing the disease severity at different stages.
PRIMER INFORME DE INFECCIÓN NATURAL POR Platynosomum spp. EN GATO DOMÉSTICO EN EL MUNICÍPIO DE FORTALEZA, CEARÁ, BRASILRESUMEN: El Platynosomum spp. es un trematodo que habita en los ductos biliares y en la vesícula biliar de gatos, que se encuentran en todo el mundo en áreas con climas tropicales y subtropicales. Los gatos que viven en estas áreas se infectan al ingerir los vertebrados inferiores, como los lagartos y las ranas. Muchos casos de platinosomosis son asintomáticos, sin embargo, gatos con infecciones graves pueden presentar ictericia generalizada, vómitos, diarrea mucoide, anorexia, pérdida de peso, depresión y la muerte. Los signos clínicos no son específicos y pueden pasar desapercibidos, dificultando así su control y tratamiento. El objetivo de esta investigación ha sido reportar el primer caso confirmado de esta enfermedad en gato domés-tico ocurrido en el municipio de Fortaleza, Ceará. Por lo tanto, se puede concluir que platinosomosis debe ser investigado en gatos de la región, que presenten señales característicos.
Canine endocrinopathies, such as hypothyroidism and hyperadrenocortism,induce typical dermatological alterations. Collagen fibers are significant for the maintenance of structural integrity,as well as in the determination of tissue function. This study aimed at assessing the coloration caused by Picrosirius Red staining under circular polarization and Masson Trichrome staining, as tools to quantify the total collagen in the skin of dogs exhibiting endocrine dermatopathies. Skin samples taken from dogs with hypothyroidism and hyperadrenocorticism were stained using Hematoxylin and Eosin (HE), Masson’s Trichrome (MT) and Picrosirius Red (PSR). The animals with hyperadrenocorticism revealed a higher percentage of collagen area than did the animals with hypothyroidism PSRp (hypothyroidism = 43.76 ± 0.8255 and hyperadrenocorticism = 47.08 ± 0.8584). The percentage of the collagen area using MT between the groups is given in parenthesis (hypothyroidism = 52.06 ± 0.9371, hyperadrenocorticism = 61 ± 0.7529 and control = 56.88 ± 0.64) (p <0.05). It is therefore, evident that the special stains employed are useful in estimating the percentage of collagen area in the skin, as they revealed that dogs with hypothyroidism had lower collagen deposition whereas those animals with hyperadrenocorticism showed higher quantity of collagen in the dermis.
The present study evaluated the effect of Ovarian Tissue Cryosystem (OTC) on follicular morphology and density, as well as on stromal cell density of vitrified canine ovarian tissue. Canine ovarian fragments collected from adult female dogs in stages of the random oestrous cycle were fixed (FC, fresh control
Snakes have an important biological role in the ecological chain, as well as in scientific researches performed with the venoms produced by them, since the enzyme-protein fractions these substances possess have been studied as pharmacological tools for the discovery of new therapies. Snakes of the genus Crotalus have gained significant relevance in the scientific field, since the venom produced by these reptiles has been the target of researchers in a few decades, due to the composition and the effects that these substances can produce. In Brazil, a single species represents the genus, which is Crotalus durissus. This review demonstrates that even with the advancement in scientific research on the composition, role and application of the venom produced by the subspecies of Crotalus durissus snake, it is necessary to further study their fractions and their action potential, which also demonstrates the how rich are these active components in different fields of biomedicine.
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