Background: Pseudomonas aeruginosa is one of the top fi ve pathogens causing healthcareassociated infections. Biofi lm formation is nowadays a major problem. Aim: The aim of this study was to examine the prevalence of virulence genes in clinical isolates of Pseudomonas aeruginosa at Suez Canal University Hospitals with respect to the site of infection and microbial resistance of the strains.
Materials and methods:A cross-sectional descriptive study was carried out on 47 Pseudomonas aeruginosa strains collected from hospitalized patients from December 2015 to August 2017. To detect biofi lm formation, we used Tissue Culture Plate Method. The virulence genes (toxA, algD, nan1, pslA and pelA) were amplifi ed using PCR technique.
Results:The highest sensitivity was to Imipenem and Ciprofl oxacin (85.1% and 68.1% respectively).With respect to the virulence genes, toxA gene was detected in 45 isolates (95.7%), algD gene in 42 isolates (89.4%), pslA in 42 isolates (89.4%) %), pelA cted in 41 isolates (87.2%) and nan1gene was detected in 19 isolates (40.45%).
Conclusions and Recommendations:We conclude that there is relationship between virulence genes and biofi lm formation in Pseudomonas aeruginosa. We recommend the expansion of work on a larger sample size in a longer period of time.
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