1 Acetylcholine caused a concentration-dependent smooth muscle hyperpolarization and relaxation in rat small mesenteric arteries (diameter at 100 mmHg 250-450 mm) stimulated with noradrenaline (3 jM). 2 Nitric oxide (NO), generated from either NO-gas or from acidified sodium nitrite, also induced smooth muscle hyperpolarization but only in the absence of active force. However, unlike the hyperpolarizations to acetylcholine, those to NO were abolished either by prior smooth muscle depolarization caused by noradrenaline, or by the K+ channel blocker, glibenclamide (3 gM).3 Hyperpolarization and relaxation to acetylcholine were unaffected by prior exposure of the mesenteric artery to either the cyclo-oxygenase inhibitor, indomethacin (10piM), or the nitric oxide synthase inhibitor, NG-nitro-L-arginine (L-NNA, 100pUM). 4 Haemoglobin (1.5 pM), which binds and inactivates NO, blocked the hyperpolarizing and vasorelaxant response to NO, but did not alter either response to acetylcholine. 5 These data show that, in the rat small mesenteric artery, membrane hyperpolarizations to NO and acetylcholine are mediated by different mechanisms, and that the hyperpolarization induced by NO is not involved in the responses to acetylcholine. In addition, they provide evidence that the acetylcholine responses in this artery, which are endothelium-dependent, are not mediated by the release of NO.

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The purpose of this paper is to know how the string sight-reading is nowadays being practiced and being taught in the Spanish music education system. The relation between the use of sight-reading tests in exams and the previous preparation of students to succeed, is being analyzed. Studies in different fields of sight-reading are reviewed as well as conservatory study plans and Spanish regulations, in the different regions, about sight-reading planification and exams. The English system, ABRSM has been studied to establish a comparison with Spanish music education