The data suggested a potential application of SELDI-TOF MS as an effective technology to profile serum proteome, and with pattern analysis, a diagnostic model comprising three potential biomarkers was indicated to differentiate people with TB and healthy controls rapidly and precisely.
In the rice-wheat rotation system, 24 and 23 wheat cultivars were separately planted in Yangzhou and Suining of Jiangsu province in 2016-2017 and 2017-2018. According to nitrogen utilization rate (NUR), these cultivars were clustered into three groups, i.e., NUR-H (NUR ≥ 50%), NUR-M (NUR 40%-50%), and NUR-L (NUR ≤ 40%), to identify the differences in grain yield, yield components, and population quality, which would provide a reference for cultivar selection for high-yield and high-efficiency in wheat production. Yangmai 25 and Ningmai 21 in Yangzhou and Huaimai 35 in Suining showed NUR-H phe
Background & AimsStudies have documented cellular apoptosis, autophagy and endoplasmic reticulum (ER) stress participate in the liver fibrogenesis. However, the specific mechanism remains unclear. This study was aimed to determine whether and how eIF2α protein play a role in the collagen generation in hepatic stellate cell (HSC) via autophagic signaling events.MethodsHSC‐LX2 cell line was activated by transforming growth factor beta1 (TGF‐β1) in the presence of eIF2α plasmid or vehicle control. Real‐time fluorescence quantitative PCR was then performed to compare the differential mRNA level of COL1, eIF2α, CHOP, Bcl‐2 and Bax. Western Blot was performed to analyze the protein expression of α‐SMA, COL1, LC3, P62, eIF2α, P‐eIF2α and Bcl‐2 in LX2 cells. Meanwhile, the numbers of apoptotic cells were measured by flow cytometry.ResultsCompare to vehicle control, the ER stress hallmarks of eIF2α, CHOP were at lower level in HSC‐LX2 cells in TGF‐β1 treated condition. While transfected eIF2α plasmid in HSC‐LX2 cells, it was found that CHOP and BAX significantly increased in these cells but substantial reduction in LC3, P62, Bcl‐2, α‐SMA and COL1 v.s. control(<0.05). Moreover, thus exogenously enhancing of eIF2α gene was found to induce apoptosis of HSC‐LX2 cells with a 50% increment (<0.05).ConclusionsOverexpression of eIF2α in HSCs may trigger autophagy inhibition and apoptosis promotion, thereby reduce collagen production in these cells, which provide a potential therapeutic strategy for hepatic fibrosis.Support or Funding InformationSupported by NSFC Grants 81360077, 81860654, 30630145, 81172260 and GXNSFC Grants 2015GXNSFDA139022 to Guo ZhangThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
The experimental study of laser-driven material state equation puts forward extremely high requirements for the uniformity and stability of the target spot intensity distribution, and these two characteristics greatly determine the accuracy and repeatability of the experimental results. In this paper, a beam smoothing scheme combining diffraction-weakened lens array (LA) with induced spatial incoherent (ISI) technique based on low-coherence laser is proposed to solve the problems, that is, the uniformity and stability of the target spot intensity distribution in the material state equation experiments driven with narrow-band coherent laser. The super-Gaussian soft aperture used in our scheme can improve the intensity fluctuation caused by the hard-edge diffraction of the lens elements, and the temporal smoothing technique, ISI, can reduce the interference effect between the lens array elements. The speckle patterns of target spot, which are caused by interference between beamlets and determine the high nonuniformity, will randomly reconstruct after each coherent time. The high-frequency components are further smoothed by the time-average effect. In broadband high-power laser devices, ISI can be combined with LA by making the lens elements with different thickness values. This scheme can enhance the focal spot uniformity and improve the tolerance of the system to the wavefront phase distortion. The influence of wavefront phase distortion on target surface uniformity and stability are analyzed. The simulation results show that this smoothing scheme significantly reduces the target spot nonuniformity, improves the tolerance of random wavefront phase distortion, and presents a uniform and stable target spot intensity distribution. The nonuniformity of target spot will be reduced to about 10% after 10 ps, and about 3% after 100 ps. In addition, statistical analysis shows that the peak-to-valley value and the nonuniformity of the target spot intensity distribution are strongly correlated with the gradient of root-mean-square of the wavefront phase distortion. Using this method, the tolerance range of the wavefront phase distortion can be given according to the requirements of the experiments, which has reference value for designing and optimizing the laser driver parameters in the state equation experiment.
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