B. Nmr Spectrum Investigations. (1) Nmr Spectrum of Chlorophyll Used in Kinetic Studies. The chlorophyll (40 mg) was dissolved in chloroform-t/ (ca. 0.3 ml) and the spectrum determined. It proved to be virtually the same as that previously reported.6(2) Reaction of Chlorophyll with Amines in Tetrahydrofuran.The chlorophyll (ca. 35 mg) was dissolved in about 250 ml of tetrahydrofuran. The amine (20-25 ml) was added and the solution sealed on a vacuum line after degassing. The reaction was usually complete after 1 day. The tube was opened, the contents transferred, and solvent and excess amine were removed in vacuo.Chromatography on powdered sugar as described above gave the amides.
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