A recently developed model for pretreatment and acid or alkaline hydrolyses of lignocellulosic biomasses, including blue agave bagasse (BAB), in twin-screw corotating extruders is extended to the case where enzymes are incorporated into the formulation. The model describes the deconstruction and degradation of BAB, as well as the production of simple sugars at the end of the extrusion process. Experiments using NaOH for delignification and alkaline hydrolysis of BAB, H 3 PO 4 for neutralization, and different enzymatic cocktails for bioextrusion are reported. The effects of temperature, NaOH concentration, enzymatic cocktail used and its concentration, residence time, and configuration of screw elements on degree of degradation and production of sugars are assessed. It turned out that temperature, NaOH concentration, and enzymatic cocktails are the key factors in the extraction and hydrolysis of hemicellulose (H) and cellulose (C) to produce glucose (G). Good agreement between experimental data and model predictions is observed.
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