A water deficit in the soil can cause water stress in plants, triggering morphological and physiological changes. The aim of this work was evaluate the ecophysiological development of moringa seedlings under controlled water restriction. The experimental design was completely randomized at 40, 60, 80, and 100% of field capacity and six replicates. The photosynthetic CO 2 assimilation, stomatal conductance, transpiration, vapor pressure deficit, internal carbon concentration, chlorophyll a, chlorophyll b, and total chlorophyll and stem diameter, height, and number of leaves were measured at 9 a.m. during 21 days of restriction. The treatments differed for photosynthetic parameters. Moringa seedlings reduce gas exchange to adapt to water restrictions until 40% of field capacity. The alterations promoted by water restriction did not negatively affected plant development.
Hancornia speciosa Gomes, popularly known as mangaba tree, is a fruit tree native to Brazil, with natural occurrence in several regions. However, some factors have contributed to the reduction of natural populations of this species, in addition to the recalcitrant characteristic of its seeds, which hinders their storage for conservation purposes. The application of plant tissue culture techniques is a complementary strategy to the conservation of the existing genetic variability and allows Original Research Article
This study evaluated the effect of vitrification solutions and exposure time on the cryopreservation of Brazilian green dwarf coconut plumules (BGD) using the droplet vitrification technique. Explants were excised from BGD mature fruits from the Active Germplasm Bank of Embrapa Tabuleiros Costeiros, Sergipe, Brazil. Firstly, embryos were disinfected, and after excision, plumules were pre-cultivated for 72 hours in Y3 + 0.6 M sucrose + 2.2 g L -1 Gelrite ® culture medium. Plumules were exposed to PVS2 and PVS3 solutions for 15 and 30 minutes and rapidly immersed in liquid nitrogen (-196 ºC). After cryopreservation, they were thawed in culture medium solution (Y3 + 1.2 M sucrose) and cultured in regeneration medium. The experimental design was completely randomized in a 2x2 factorial scheme (vitrification solutions per exposure times), with five replicates per treatment. Data were compared by the Tukey's test at 5% probability. Significant differences were observed in the callogenesis percentage for the solutions x exposure time interaction for non-cryopreserved cultures (-NL) and for exposure time after cryopreservation (+NL). PVS2 and PVS3 combined with 15 minutes of exposure promoted the highest callus formation (70 and 100%, respectively) in control cultures. The exposure time of 30 min, regardless of vitrification solution, resulted in 30% embryogenic callus formation after cryopreservation. These results contributed to the long-term conservation of coconut palm.RESUMO: O objetivo desse estudo foi avaliar o efeito das soluções de vitrificação e do tempo de exposição na criopreservação de plúmulas de coqueiro anão verde do Brasil de Jiqui (BGD), pela técnica de vitrificação em gotas. Os explantes foram excisados de frutos maduros oriundos do Banco de Germoplasma Ativo de Embrapa Tabuleiros Costeiros, Sergipe, Brasil. Os embriões foram desinfestados e as plúmulas, após a excisão, pré-cultivadas durante 72 horas em meio de cultura Y3 suplementado com sacarose 0,6 e 2,2 g L -1 Gelrite ® . As plúmulas foram expostas em soluções de PVS2 e PVS3 durante 15 e 30 minutos, e rapidamente imersas em nitrogênio líquido (-196 ºC). Após a criopreservação, foram descongeladas na solução de meio de cultura Y3 com 1,2 M de sacarose, e cultivadas em meio de regeneração. O delineamento experimental foi inteiramente casualizado em esquema fatorial 2x2 (soluções de vitrificação x tempos de exposição), com cinco repetições por tratamento. Os dados foram comparados pelo teste de Tukey à probabilidade de 5%. Observaram-se diferenças significativas na porcentagem de calogênese para a interação entre soluções e tempo de exposição para as culturas não criopreservadas (-NL), e para o tempo de exposição após a criopreservação (+NL). O PVS2 e o PVS3 combinados com 15 minutos promoveram a maior formação de calo (70 e 100%, respectivamente) nas culturas de controle. O tempo de exposição de 30 min, independente da solução de vitrificação, promoveu 30% da formação de calos embriogênicos após a criopreservação. Estes resultados contribuem para ...
Azadirachta indica A. Juss, popularly known as neem, is a species native to India, belonging to family Meliaceae, considered the most important plant species with insecticidal action. The aim of this study was to evaluate the influence of growth regulators on induction and growth of neem callus and to observe their viability for embryogenesis through morpho-histological characteristics. In vitro germinated plants were used for excision of nodal explants. These segments were inoculated in Murashige and Skoog culture medium containing 1.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic) combined with BAP (6-benzylaminopurine) at the following concentrations: 0.0, 0.5, 1.0 and 2.0 mg/l (T1, T2, T3 and T4 respectively), for callus induction. At 0 (mass of nodal segments without callus), 20, 40 and 60 days of culture, the percentage of callus formation was observed and the callus weight was measured for each treatment and at the end of the 60 days, consistency, color, and cell histology were evaluated. There was callus formation in all treatments tested. The highest induction of Azadirachta indica A. Juss callus is observed in the presence of 1.0 mg/l 2,4-D + 2.0 mg/l BAP, with callus showing light brown color, friable consistency and rounded cells with intense cell division, typical of cells with potential embryogenic capacity.
Abiotic stresses in plants have aroused great research interest, unceasingly seeking for more productive cultivars under unfavorable cultivation conditions. Water deficiency and soil salinity are the most studied aspects, due to their strong impact on the growth and productivity of plants. The objective of this study was to evaluate accessions of different sugarcane species (Saccharum spp., S. robustum, S. officinarum) regarding tolerance to in vitro salinity, from changes in physiological, anatomical, biochemical and biometric parameters, to assist in breeding programs. To this end, shoots were used, grown on Murashige and Skoog medium, supplemented with 2% of sucrose and 4 g.L-1 Phytagel, in the presence of five concentrations 0, 50, 100, 150, 200 mM NaCl. Shoots length, roots length, shoots number, roots number, fresh weight, dry weight, proline content in leaves, total chlorophyll content, and morphological changes to the leaf tissue were analyzed. The in vitro growth of accessions Saccharum robustum, Saccharum spp. and Saccharum officinarum are affected by salinity induced by NaCl. The proline accumulation and chlorophyll decrease are intensified in Saccharum spp., in addition to changes in cell's anatomy, characterized as more sensitive to salt.
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