Two synthetic peptides comprising an RGD moiety for intecase of the COS-7 and A375M cell lines. With the ECV304 grin binding and a polylysine moiety for DNA binding were cell line, optimum transfection occurred in the absence of tested for transfection efficiency under a variety of different chloroquine. Transfection efficiency of the peptides was conditions. Binding of target cells to the peptide was shown greatest at peptide:DNA ratios of 4:1 (w/w), which were to be strongly dependent on cyclisation of the peptides via calculated to generate complexes containing approxicysteine residues. Low (10 M) concentrations of chloromately 5000 peptide molecules per plasmid. This repquine, added to assist endocytic exit, unexpectedly resented approximately a 6:1 ratio of positive to negative reduced transfection efficiency in two of the cell lines charges. Peptide 5 was shown to have a higher transfectested, COS-7 and ECV304. However, transfection tion efficiency under most conditions, possibly because of efficiency increased at higher chloroquine concentrations more efficient stabilisation of cyclisation by two cysteineand exceeded that in the absence of chloroquine in the cysteine bonds.
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