BackgroundConsidering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted onto the gingival margin regarding cytokine secretion.MethodsBiopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were isolated by the explant technique, cultured and stimulated with Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for 24 or 48 h for comparative evaluation of the secretion of cytokines and chemokines, such as IL-6, IL-8/CXCL8, MIP-1α/CCL3, TGF-β, VEGF and CXCL16. Unstimulated cells were used as the control group. Cells were tested for viability through MTT assay, and secretion of cytokines and chemokines was evaluated in the cell supernatants by Enzyme-Linked Immunosorbent Assay (ELISA).ResultsFibroblasts from the palatal mucosa maintained the same secretion pattern of IL-6 when grafted onto the gingival margin. On the contrary, fibroblasts from the marginal gingival graft showed increased secretion of IL-8/CXCL8 even in the absence of stimulation. Interestingly, MIP-1α/CCL3 secretion by fibroblasts from the marginal gingival graft was significantly increased after 48 hours of stimulation with Pg LPS and after 24 h with Ec LPS. Only fibroblasts from the marginal gingival graft showed secretion of TGF-β. VEGF and CXCL16 secretion were not detected by both subsets of fibroblasts.ConclusionFibroblasts from the palatal mucosa seem to be adapted to local conditions of the site microenvironment when grafted onto the gingival marginal area. This evidence supports the effective participation of fibroblasts in the homeostasis of the marginal periodontium through secretion modulation of important inflammatory mediators.
Análise da sobrevivência de próteses metalocerâmicas após um período mínimo de quatro anos em funçãoshowed that the success rate of metal-ceramic prosthesis was 87.8% and the survival rate was 89.1%. In addition, the success rate was independent of age (p=0.903), installation time (p=0.830), number of prosthesis (p=0.872), elements (p=0.937) and abutments (p=0.064). The qualitative variables also did not show significant statistical results (p> 0.05) between success and failure rates. However, the results showed that patients who did not use myorelaxant plaque had a higher success rate than plaque users (p=0.004). Thus, it can be concluded that the metal-ceramic prosthesis has high success and survival rates, ensuring longevity of this type of rehabilitation.
Objetivo: Analisar o contexto atual a respeito da relação bidirecional entre doença periodontal e diabetes mellitus. Métodos: Levantamento bibliográfico de artigos publicados entre os anos de 2018 a 2022, utilizando os descritores "diabetes mellitus" e "doença periodontal", nos idiomas português e inglês, disponíveis na íntegra, encontrados nas bases de dados LILACS, MEDLINE e BBO, sendo estudos de revisão sistemática, ensaio clínico randomizado, estudos coorte, série de casos ou estudos transversais. Resultados: Foram encontrados um total de 137 artigos, sendo que, pelos critérios de inclusão utilizados apenas 8 artigos foram selecionados. Dentre esses artigos, a maioria foram do tipo revisão sistemática, onde foi possível analisar a relação bidirecional entre a doença periodontal e o diabetes mellitus e notar-se a importância do acompanhamento multidisciplinar para a não progressão de ambas as doenças. Considerações finais: O paciente diabético tem predisposição à doença periodontal, assim como, a doença periodontal interfere no nível glicêmico do paciente diabético, evidenciando a relação bidirecional entre elas.
Introdução: A hipersensibilidade dentinária é uma dor aguda e rápida e acomete diversos pacientes tendo uma etiologia multifatorial e entre as principais causas cita-se o uso de aparelho ortodôntico que também é um fator de risco para recessão gengival (RG), especialmente, em pacientes com fenótipo periodontal delgado. Objetivo: o presente trabalho tem como objetivo relatar um caso de RR múltiplo na região látero-inferiorutilizando enxerto autógeno em área deLCNCs.Relato de Caso: Paciente do sexo masculino, 32 anos de idade, não tabagista, nem etilista e sem alterações sistêmicas e sem alergias prévias foi encaminhado para uma clínica odontológica de um curso em periodontia, na cidade de Belém, norte do Brasil com a queixa principal de HD na região látero-inferior. No exame clínico verificou-se RG tipo 1 (RT1) múltiplas, nos dentes 43, 44 (presença de lesão cervical não cariosa) e 45, presença de fenótipo periodontal fino e festonado, sem perdas ósseas interproximais, ausência de sangramento a sondagem, biofilme e cálculo dentário.O tratamento indicado foi o recobrimento radicular pela técnica de deslize coronal associado ao enxerto autógeno para melhoria do nível clínico de inserção e aumento da faixa de mucosa queratinizada com MDA fixada com suturas. Conclusão: Diante do exposto, verificamos que os resultados clínicos relatados neste trabalho indicaram um ótimo resultado clínico para aumento da faixa de mucosa queratinizada, ganho clínico de inserção e resolução da HD.
Objective Metal-ceramic prosthesis are the treatment of choice in oral rehabilitation because of their high survival rates. However, there are few reports in the scientific literature about factors that lead to fractures of metal-ceramic prostheses. The aim of this study was to evaluate whether prostheses units number, abutment type and number, type of the prostheses, prostheses position in the mouth, bruxism and occlusal plaque influence the incidence of fractures in fixed metal-ceramic prostheses. Methods For this study, 16 patients were selected, totaling 74 metal-ceramic prostheses installed between 2000-2010, with follow up of at least four years. Besides dental history, other information was collected, such as patients’ gender and age, prostheses installation date, and the ceramic system used. In case of prostheses fracture, a questionnaire was applied to identify signs of bruxism. A clinical evaluation was done to evaluate the prostheses integrity and opposing dentition characteristics. Success, failure and survival rates were determined. Results The results showed that the success rate of metal-ceramic prostheses was 87.8% and the survival rate was 89.1%. In addition, the success rate was independent of patient age, prostheses installation time, number of prosthesis, number of prosthesis’ units and abutments. The qualitative variables also did not show significant statistical results between success and failure rates. Conclusion It was concluded that metal-ceramic prostheses have high success and survival rates, what guarantees longevity and indication of this type of prostheses in daily practice.
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