Shiga toxin producing E. coli is represented as one of the main source of foodborne infectious disease distributed all over the world. This area of study not fully explained before at Ismailia governorate. So this study aimed to make genetic survey of all isolated E. coli strains with attention to shiga toxins isolated from different sections of poultry slaughterhouses and workers at Ismailia governorate. One hundred and fifty swab samples were collected from baskets, workers hands, machines, processing tools and food contact surfaces and subjected to bacteriological examination. Ten samples were positive for E. coli with a percentage 6.6%.
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