Conception and design of the study, technical procedures, acquisition and interpretation of data, technical procedures, manuscript preparation.

This study performed a genetic study of several canopies of Anacardium occidentale L. specimen called "Cashew King" (Cajueiro da Praia-PI, Brazil) in order to assess whether all canopies belong to the same plant. Leaves were collected from

Assessments of chromosomal integrity and structure enable the prevention of diseases associated with the work environment, with the frequencies of chromosomal aberrations and micronuclei often being used as markers in biomonitoring. Owing to their routine manipulation of potentially toxic chemicals, tannery workers as a group merit a more thorough evaluation and discussion. This study investigated chromosomal damage in 30 workers from a tannery in the city of Teresina, the state capital of Piauí, northeast Brazil, and a control group consisting of 30 employees from a nearby accounting firm. The frequencies of chromosomal aberrations (CAs) and binucleated cell micronuclei (MN) were assessed as a measure of damage. Means were compared using the Student t-test and ANOVA-Dunnett test. Our results indicated a higher number of CAs in exposed individuals compared to the control group, including dicentric (P < 0.0001) and tricentric chromosomes (P < 0.001), and those in ring (P < 0.0001) and acentric ring forms (P < 0.001). Assessment of MN frequency demonstrated a similar trend (exposed vs control, P < 0.0001). It was concluded that the tannery workers in this study exhibited a higher incidence of genetic damage than comparable unexposed individuals. However, further research on this subject is needed, particularly in regard to potentially clastogenic agents used in the tanning process.

Objective: In this scientific study, we aimed to evaluate genotoxic effects in rats (Rattus norvegicus), related to different periods of exposures to the LED curing light. Methodology: For the genotoxicity evaluation, the rats received lights from the LED photopolymerizer for 40 sec, 10 min and 7-and-a-half minutes, while the negative and positive control groups were treated with distilled water and cyclophosphamide by intraperitoneal, respectively. A sample of peripheral blood was collected from the animals for the comet assay. The bone marrow was collected from each rat for the micronucleus test. Results: It was observed that in the comet assay and micronucleus test, the animals exposed to LED for 10 min, showed genotoxic damage, and they have not presented toxicity degree in the periods of 40 sec and 7-and-a-half minutes. Conclusion: It is possible to conclude that, there was genotoxic effects on the animals' teeth when exposed to the LED curing light in 10 min. However, in the periods of 40 sec, and 7- and-a-half minutes, have been not observed genotoxic effects. This means these times are safe for professional dentists in clinical care.