Irisin was first identified in muscle cells. We detected irisin immunoreactivity in various organs of the crested porcupine (Hystrix cristata). In the epidermis, irisin immunoreactivity was localized mainly in stratum basale, stratum spinosum and stratum granulosum layers; immunoreactivity was not observed in the stratum corneum. In the dermis, irisin was found in the external and internal root sheath, cortex and medulla of hair follicles, and in sebaceous glands. Irisin immunoreactivity was found in the neural retina and skeletal muscle fibers associated with the eye. The pineal and thyroid glands also exhibited irisin immunoreactivity.
Background: Previous researches about the effects of epididymal obstruction on the testes are contradictory, and the mechanism harmful effect of male duct system obstruction on seminiferous tubules still remains unclear.
Objective: The aim of this study was to investigate the effects of epididymal obstruction in prepubertal rats on the testis.
Materials and Methods: 15 days of age, the young rats were divided at random in two groups for epididymal ligation (n=25) or sham operation (n=15). Both groups were sacrificed at 21, 35, 56, 90, 120 days. The testis were removed, fixed in Bouin’s fixative and embedded in paraffin wax. The tissues were sectioned at 5 µm and stained with haematoxylin-eosin and triple stain.
Results: In ligated rats the first histological alterations were detected at 56 days. These degenerative changes included increase at the seminiferous tubule diameter and basal membrane thickness, decrease at the germinal epithelium thickness, depletion of spermatids and presence of multinucleated spermatids. In 90 and 120 days ligation groups; germ cells greatly reduced in number.
Conclusion: progressive degenerative alterations occurred in the seminiferous tubules after prepubertal epididymal obstruction but these degenerative alterations are not observed until puberta and in the seminiferous tubules that showed extensive degeneration, seminiferous epithelium was composed mainly of Sertoli cells.
ABSTRACT:To better understand the effect of androgen on the growth and function of accessory sex glands, it can be useful to determine the presence or absence of the androgen receptor (AR) in the individual cell types. Five adult intact male rats were sacrificed at 120 days. Their seminal vesicles and prostate glands were removed, fixed in Bouin's fixative and embedded in paraffin wax. The tissues were sectioned at 5 μm and stained using the microwave-stimulated antigen retrieval technique for immunohistochemistry. Positive immunohistochemical staining for the AR was evident in nuclei but not in the cytoplasm of gland cells such as luminal cells, basal cells, periacinar smooth muscle cells, other stromal cells, and smooth muscle cells in the tunica muscularis. The proportions of AR-positive and AR-negative basal cells were similar. The staining intensity of luminal cells was greater than basal cells and stromal cells. The numbers of AR-positive and AR-negative luminal cells in the prostate were almost equal. On the other hand, in the seminal vesicle only a small number of luminal cells were AR-negative. These observations can be interpreted to mean that the epithelium of the seminal vesicle is more sensitive to androgenic stimulation than the prostate epithelium.
Androgens exert their effects through androgen receptors (AR) in tissues. We investigated the distribution of AR in female mole rat tissues. Tissues were excised, fixed with 10% formalin and embedded in paraffin. Sections were stained after microwave antigen retrieval for immunohistochemistry. Immunostaining of AR immunostaining was detected in the nucleus or cytoplasm of the cells in the cerebral cortex, cerebellum, anterior pituitary, lung, liver, uterus and skin. Granulosa and some thecal cells in the ovary, cardiac muscle cells and adipose cells exhibited a nuclear reaction for AR. In the kidney, labeling of AR was restricted to the cytoplasm of tubule cells. We found that AR could be detected using immunohistochemistry in the nucleus or cytoplasm or both in the presence of androgens.
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