The shelf life of seafood under current icing and refrigerated storage conditions ranges from 2 to 14 d, depending on species, harvest location, and season. Elevated carbon dioxide levels in modified atmosphere packaging (MAP) has been shown to inhibit the normal spoilage flora of seafood and double or triple shelf life. The threat of botulism, due to the presence of nonproteolytic psychyrotrophic Clostridium botulinum types B, E, and F, has been reason for caution in expanding this technology. This article examines the safety of MAP seafood through analysis and comparison of raw materials, research methodologies, quality indices, treatment, and packaging options.

Cut romaine lettuce, packaged under modified atmosphere, was subjected to 0.15 and 0.35 kGy gamma irradiation. Irradiation at 0.35 kGy decreased aerobic plate counts by 1.5 logs and yeast and mold counts by 1 log; these differences were maintained through the 22-d storage. Irradiation at 0.15 kGy caused smaller reductions in microbial counts. A decrease in headspace CO 2 was observed in the 0.35-kGy-treated lettuce, although CO 2 concentrations increased in all samples with storage. O 2 concentration was not affected by irradiation. Ten percent loss in firmness was observed at 0.35 kGy, while other sensory attributes such as color, generation of off-flavor, and appearance of visual defects were not affected.

The effects of 0.5 and 1.0 kGy gamma irradiation on microbial and sensory characteristics of diced celery were compared to conventional treatments such as acidification, blanching, and chlorination. Aerobic plate counts for the irradiated celery did not exceed 10 7 CFU/g, in contrast, the acidified, blanched, chlorinated, and control samples surpassed aerobic microbial counts of 10 8 CFU/g in 22, 19, 12, and 8 d, respectively. Importantly, the 1.0-kGy treatment eliminated inoculated Listeria monocytogenes and E. coli. While the acidified and blanched samples had significantly different sensory profiles compared to the other treatments, the irradiated samples maintained their color, texture, and aroma longer and were preferred in the sensory tests. The sensory shelf life of the 1.0-kGy-treated celery was 29 d compared to 22 d for the control, chlorinated, and 0.5 kGy, and 15 d for the acidified and blanched celery.

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