Aim: The aim of this study is to compare whether there are differences in the value content of secondary metabolites (saponins, tannins, and flavonoids) between the group treated with Binahong leaves (administration of colchicines) and the group treated with Binahong leaves without the application of colchicines.
Materials and methods:The design of this study is an experimental research laboratory study. This research was done in four different places in which colchicines were applied to improve the quality of Binahong, conducted in the USU Agricultural Faculty's Greenhouse, whereas no application of colchicines was done in the Desa Simpang Pergendangan Kelurahan Tiga Binanga Kabupaten Karo harvesting Binahong plant. The pharmacy faculty of USU was chosen as a place to obtain the extract and the Laboratory of the Research Institute for Spices and Medicinal Plants (Balitro) was where the phytochemical analysis was conducted.
Results:The results showed that there were differences in the descriptive values of secondary metabolites in the Binahong leaf extract, such as saponins, tannins, and flavonoids, wherein the group with colchicines was higher than groups without colchicines, but there was no statistical differences between the groups treated with colchicines and those not treated with colchicines (value p > 0.05).
ORIGINAL RESEARCH
10.5005/jp-journals-10015-1453Conclusion: Administration of colchicines can improve the content of secondary metabolites in Binahong leaf extract and the plant morphology without affecting the content of the plant extract.Clinical significance: Secondary metabolites in herbal plants, such as saponins, flavonoids, and tannins are able to speed up the healing process. One of the plants that have all three of these active compounds is Binahong (Anredera cordifolia). Binahong leaf extract can stimulate the process of fibroblasts and collagen formation that will accelerate the process of wound healing. Colchicine as a reagent for the mutation is able to affect the number of leaves and morphological characteristics of the Binahong plant.
The morphological and biochemical performance of red rice (Oryza sativa L.) in Munte and Payung Subdistricts in Karo District. The aim of this research is to obtain morphological information and anthocyanin content of red rice (Oryza sativa L.). Descriptive survey method using International Rice Research Institute (IRRI) guidance.Location determination using purposive method and accidental technique for sampling. Anthocyanin content test using analytical method by homogenizing the sample. Exploration results obtained 72 genotypes are divided into three land locations. Based on anthocyanin content test obtained A land has the highest content of 0.5mg / 100g and the lowest is B land 0.08mg / 100 g. ABSTRAK Keragaan morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.) pada Kecamatan Munte dan Kecamatan Payung di Kabupaten Karo. Penelitian ini bertujuan untuk mendapatkan informasi morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.). Penelitian ini dimulai dari Juni 2017dan selesai pada Januari 2018 di kecamatan Munte dan Kecamatan Payung Kabupaten Karo.Metode survei deskriptif menggunakan panduan International Rice Research Institute (IRRI). Teknik penentuan lokasi secara sengaja dan pengamblan sampel secara kebetulan.Uji kandungan antosianin menggunakan metode analitik dengan menghomogenkan sampel. Hasil eksplorasi didapatkan 72 genotipe yang dibagi menjadi tiga lokasi lahan. Berdasaran uji kandungan antosianin didapatkan lahan A memiliki kandungan tertinggi yaitu 0,5 mg/100 g dan terendah yaitu lahan B 0,08 mg/100 g.
Soybean is one of the leading strategic food crop in Indonesia. Nowadays, people's need for soybeans is not only seen from the production aspects but also soybean nutrient contents, especially fatty acids. One of the ways to develop superior varieties of soybean with high fatty acids is through mutations. Seven soybean mutants selected M5 and M7 generation with high production character and resistance Athelia rolfsii Curz were genetically related to high fatty acids using SSR markers. DNA quality and quantity test results showed DNA purity between 1.12-2.13 with concentration between 15.4-97.9 μg/mL. The FAD2-2D locus is the gene candidate of the result by the QTL mapping used in this study to identify soybean genes related to linoleic acid characters. The results showed that the primer used was specific and polymorphic. Mutant soybeans tested have a high genetic diversity.
This study aims to determine the growth and production response of some mung bean varieties with the application of gibberellin at different concentrations in the saline field. This research was conducted at Hamparan Perak , District of Deli Serdang North Sumatera, Medan (± 3-9 m asl) from May - August 2017. The tretment was arranged by Randomized Block Design (RBD) with two factors, three mung green varieties (Vima-1, Vima-2, and Vima-3) and GA3 concentration (0 ppm, 100 ppm, 200 ppm). The results showed that there were significant diffrent among varieties on plant height, the number of pods per plant, and the diameter of the seed. Gibberellin concentration gives sinificant effect to the plant height in 3 and 5 week after pllanted, root volume, and seed diameter. The interaction between aplication gibberellins and varieties had a significant effect on the root volume.
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