Introducing enzymes during the extrusion process bas been mainly used as new pretreatment techniques in the starch degradation process and, more recendy, in the second generati on bioethanol producti on. The technique, called the bioextrusion, is a special case of reactive extrusion. Starch and lignocellulose bioextrusion examples underline the good mixing capacities as a way to initiate the enzymatic reaction in high solid content conditions. Starch bioextrusion results show a low dextrinization yield but a real effect on the polymer sire decrease which all ows higher and faster subsequent saccharification. lt also considerably reduced the recrystallization phe nomenon that limits the saccharification efficiency. Bioextrusion of lignocellulose resulted in a better sugar production. Very short residence runes lirnit the use of bioextrusion to a pretreatment technique. However, unique fl exibility of the extrusion technique allows to subsequently pretreat, in the same extruder, with physical and/or chemical constraining conditions, followed by a milder bioextrusion.
Lignocellulosic biomass is a sustainable source of renewable substrate to produce low carbon footprint energy and materials. Biomass conversion is usually performed in two steps: a biomass pretreatment for improving cellulose accessibility followed by enzymatic hydrolysis of cellulose. In this study we investigated the efficiency of a bioextrusion pretreatment (extrusion in the presence of cellulase enzyme) for production of reducing sugars
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