12Vibrio tasmaniensis V. splendidus and V. neptunius species were worldwide distributed and associated 13 with aquaculture and been reported as the cause of diseases in aquatic organisms. Polyphasic analyses for 14 bacterial identification are not feasible for routine diagnostic because of the time involved. The aim of 15 this study is to design three PCR primer sets that can assist with a fast detection of these species. They 16 were designed from the 16S ribosomal RNA gene, and PCR conditions were found. Each PCR test 17 successfully identified all the tested strains of each target species. The combined specificity of V.
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