Summary
In aquatic settings, light can stimulate algal growth to affect microbial transformation of organic substrates. These effects may depend on dissolved nutrients that differentially constrain microbial autotrophy and heterotrophy to drive contrasting carbon (C) and phosphorus (P) dynamics during decomposition.
We incubated sugar maple (Acer saccharum) litter under three dissolved P amendments (0, 50 or 500 μg L−1 P) and two light levels (14 or 475 μmol photons m−2 s−1) in laboratory microcosms. We measured litter chlorophyll a, microbial respiration and net metabolism, carbon:nitrogen (C:N) and C:P content, microbial P uptake and release and litter decomposition over 134 days.
Elevated dissolved P increased algal biomass in the high‐light treatment and magnified net heterotrophy and autotrophy in the low‐ and high‐light treatments, respectively. Litter C:P and C:N declined as dissolved P increased, and litter C:P was further reduced by high light only in the highest P treatment.
Microbial P uptake fluxes peaked under moderate P and high light, whereas P release fluxes were consistently low throughout the experiment. The percent of P uptake that was released was significantly higher under low light.
High light stimulated decomposition under low P but slowed decomposition under high P, suggesting increased nutrients weakened algal priming of litter decomposition.
Our study suggests factors controlling the degree of autotrophy versus heterotrophy on organic matter, such as light and nutrient availability, may interactively shift litter C and P dynamics during decomposition.
Long-chain ω-(o-alkylphenyl)alkanoic acids (APAAs) derived from the heating of unsaturated fatty acids have been widely used for the identification of aquatic products in archaeological ceramic vessels. To date, little attention has been paid to the diagnostic potential of shorter chain (< C 20 ) APAAs, despite their frequent occurrence. Here, a range of laboratory and field experiments and analyses of archaeological samples were undertaken to investigate whether APAAs could be used to further differentiate different commodities. The results provide new insights about the conditions for the formation of APAAs and enable the proposition of novel criteria to distinguish different natural products.
Staphylococcus aureus can be carried on the skin and nasal passages of humans and animals as a commensal. A case of human methicillin-resistant S. aureus infection resulting from contact with pork has been reported. Poultry carcasses are sold at retail with the skin intact, but pork and beef typically are not. Thus, the risk of methicillin-resistant S. aureus human infection from whole raw poultry carcasses may be greater than that of exposure from pork or beef. The objective of this study was to isolate and characterize S. aureus from whole retail poultry carcasses and compare the isolates to S. aureus isolates from humans. A total of 25 S. aureus isolates were collected from 222 whole poultry carcasses. The isolates were characterized phenotypically with antibiotic resistance disc diffusion assays and genotypically using multilocus sequence typing. A total of 17 S. aureus isolates obtained from healthy humans were included and characterized in the same way as the poultry isolates. Staphylococcus spp. were recovered from all poultry carcasses. Only 25 poultry carcasses (11.2%) were contaminated with S. aureus. Of these 25 isolates, 36% were resistant to at least one of the antibiotics tested and 20% were resistant to two or more antibiotics tested. However, 100% of the human isolates were resistant to at least one of the antibiotics and 94% were resistant to two or more antibiotics. The results of the multilocus sequence typing indicate that most of the isolates grouped according to source. These results indicate a low prevalence of S. aureus present in poultry, and the isolates were not phenotypically similar to human isolates. The low number of S. aureus isolates from this study indicates that chicken carcasses would appear to not be a significant source of this bacterium.
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