To develop a simple and reliable inoculation technique using inoculum sources viz., mycelial suspension, mycelial ball, sclerotia and soil inoculation with homogenized mycelia suspension was carried out. The efficiency of different inoculation techniques were tested on susceptible rice variety Rejendra Sweta. Sheath inoculation with sclerotia gave lesion length 10.33 and 12.33 cm after seven and 15 days of inoculation, respectively. It also shows more significance in terms of relative lesion height to plant height i.e. 30.06 % followed by soil inoculation with homogenized mycelial suspension i.e. 21.62 %. However, relative number of lesions as compared to control was found to be maximum (429.18%) in mycelial suspension spray, followed by soil inoculation using homogenized mycelia suspension (400.43%). The disease rating of sheath blight of rice was also found to be maximum (5) in sheath inoculation with sclerotia followed by soil inoculation of mycelial suspension (3). The disease rating was minimum (1) in sheath inoculation with mycelial ball and foliar spray with mycelial suspension.
The potential negative effect of agrochemicals on the global environment and the cost associated with production lead to research for replacing the chemical fertilizers with bacterial inoculants. Pseudomonas fluorescens an important biocontrol agent because of its ability to induce plant growth and fungicidal/fungistatic activity. The native population of these rhizobacteria plays an important role in sustainable agriculture as they majorly dominate the rhizosphere. In the present study native, twenty-six fluorescent Pseudomonad cultures were isolated from the rhizospheric soil samples collected from the different locations of Zone 3A, Bihar and evaluated for their morphological and physiological diversity. Among the sixteen fluorescent pseudomonads tested, twelve of them produced round to oval colonies on Kings B medium. Of the sixteen isolates ascertained WHSB was produced maximum (51.33 x10‑8) number of colonies on Kings B medium followed by MSSB (28.33 X10‑8), BRKH (26.67 x10‑8) and TMSB (26.00 x10‑8). Fluorescent pseudomonad isolates varied slightly in their colony colour like green, dull-white, whitish-yellow and yellow on culture medium, the pH of 7.5 and glucose as a carbon source found best for the growth of fluorescent pseudomonads. The present work may use to develop effective formulations of potential fluorescent pseudomonads for management of soil-borne plant pathogens.
An attempt was made to evaluate the efficacy of fungicides against the false smut disease of ricecaused by Ustilaginoideavirensat farmer’s field in different locations of Bhagalpur district (Bihar) during the two consecutive kharifseasons of 2013and 2014. The results revealed that all the tested fungicides showed significantly better performance over control (Farmers practice). Technology option (TO3)seed treatment with Carbendazim @ 2g/kg seed and twospraysof Propiconazole @ 1 ml/lhas shownthe minimum disease severity (20.3%) over the all technology option and farmers practice (control). Significantly maximum grain yields (36.5 q/ha) was also obtained from technology options (TO3), which was statistically superior over TO2seed treatment with Carbendazim @ 2g/kg seed and one foliar spray of Propiconazole @1ml/l and technology option (TO1) seed treatment with Carbendazim @ 2g/kg seed and one foliar spray of Carbendazim @2g/l, whereas minimum yield (18.3 q/ha) was obtained from control
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