ABSTRACT. Zoonotic parasitic infections are a major global public and veterinary health problem and widespread among stray dogs. The objective of this study was to establish the prevalence of intestinal parasites in stray dogs in the urban, rural and coastal areas of Mexicali County in northwest Mexico. In 2014, from January to December, 380 stray dogs were captured. The entire small intestine, cecum and faeces samples were collected and examined by using simple zinc sulfate flotation and Lugol's solution staining. Data were statistically analysed. Overall, about 21.5% of examined dogs were found positive for intestinal parasites. Toxocara canis was the most frequent detected parasite, with a prevalence of 7.1%, followed by Toxascaris leonina (5.5%), Cystoisospora spp. (5.0%), Taenia spp. (3.9%) and Dipylidium caninum (2.8%). Dogs were more frequently found to be infected with a single genus of intestinal parasite (18.7%) than co-infected (2.8%). Intestinal parasites were more prevalent in samples from the coastal area (25%) than in those from the rural (24.4%) and urban (20.6%) areas, however, only statistical association was found between capture area and specific intestinal parasitic infection. There were significant differences in the prevalence of taeniasis among two age groups (P<0.01). A seasonal peak of prevalence for intestinal parasitic infections was found during spring (P<0.05), corresponding with a seasonal peak of prevalence of T. canis (P<0.05). The wide range of isolated parasites indicated that people residing in this area are at risk of exposure to these potentially hazardous zoonotic pathogens.Key words: toxocariasis, taeniasis, Mexico, public health. RESUMEN.Las infecciones zoonóticas parasitarias son un problema global público y para la medicina veterinaria, siendo diseminadas por perros callejeros. El objetivo del estudio fue determinar la prevalencia de parasitosis intestinales en perros callejeros de la zona urbana, rural y costera del municipio de Mexicali, noroeste de México. En 2014, entre enero y diciembre, se capturaron 380 perros. Se colectó el intestino, ciego y heces y se examinaron utilizando flotación con sulfato de zinc y tinción con la solución de Lugol. Los datos fueron analizados estadísticamente. En general, alrededor del 21,5% de los perros examinados fueron positivos a parásitos intestinales. Toxocara canis fue el parásito más frecuentemente, con una prevalencia del 7,1%, seguido por Toxascaris leonina (5,5%), Cystoisospora spp. (5,0%), Taenia spp. (3,9%) y Dipylidium caninum (2,8%). Los perros fueron más frecuentemente encontrados infectados con un solo género de parásito intestinal (18,7%) que coinfectados (2,8%). Las parasitosis intestinales fueron más prevalentes en muestras de la costa (25%) que del área rural (24,4%) y urbana (20,6%), sin embargo solo se encontró asociación estadística entre el área de captura y las parasitosis intestinales específicas. Hubo diferencias significativas en la prevalencia de taeniasis entre los dos grupos de edad (P<0,01). El pico esta...

Stray dogs are one of the main reservoirs of intestinal parasitic infections and some have zoonotic potential. An epidemiological survey was carried out between September 2017 and May 2018 in Mexicali Valley, this area sacrifices around 92,470 head of cattle monthly, which represents 27% of the national slaughter and has 71,307 hectares for crops. In this period the Municipal Animal Control Center during their routine visits to the Mexicali Valley captured 103 dogs. All the dogs were evaluated using copromicroscopic techniques to detect intestinal parasites. The general frequency of parasitic infections was 28.15% (29/103), the most frequent parasite being Dipylidium caninum 16.50% (17/103), followed by Taenia spp. 6.79% (7/103), Taenia hydatigena 2.91% (3/103), Taenia serialis 0.97% (1/103), Taenia pisiformis (0.97%), Toxocara canis 3.88% (4/103), Toxascaris leonina 1.94% (2/103), and Cystoisospora spp. 1.94% (2/103). No significant statistical associations were found between parasitic infections and the studied variables (sex, age, and size) however; there was a significant statistical association with the capture area. Most of the parasites found in this survey have potential to affect the human population and animal production.

El complejo respiratorio bovino (CRB) es la principal causa de enfermedad y muerte en el ganado de engorda en todo el mundo. Es un síndrome infeccioso multifactorial provocado por distintos virus y bacterias que disminuyen la eficiencia productiva y ocasionan pérdidas económicas. En México, el CRB se ha reportado en todas las regiones donde se engorda ganado; sin embargo, esos reportes carecen de información sobre la presencia del coronavirus respiratorio bovino (CVB), haciendo necesario contar con herramientas de diagnóstico confiables y precisas para detectar la presencia de CVB en el ganado que se engorda en México, para proponer las medidas sanitarias apropiadas para su manejo clínico. En este trabajo, se desarrolló una plataforma de diagnóstico molecular de PCR en tiempo real (rt-PCR) que amplifica un fragmento de la proteína S del CVB en muestras de exudado nasal. Al aplicar la plataforma rt-PCR para CVB en bovinos de engorda en aparente estado de salud y con signos de enfermedad respiratoria asociados a CRB se encontró que 19/50 (38 %) resultaron positivos, confirmando la presencia de ese virus en el ganado de la región. Los resultados de este trabajo significan el primer reporte sobre la presencia del CVB asociado al CRB en la región ganadera del noroeste de México y sienta las bases para futuras investigaciones sobre papel que juega este virus en la presentación de la patología del CRB en los sistemas de explotación de bovinos de engorda en nuestra región y el país.

The Mexican territory of the Yucatan Peninsula has a tropical climate and harbors a wide variety of domestic, synanthropic, and wild animals, as well as disease vectors. To determine the distribution of recorded zoonotic diseases in the Yucatan Peninsula, scientific publications referring to these diseases in animals and containing geographic coordinates of disease occurrence, were studied. The epidemiological bulletins of the national government were also consulted to obtain information on zoonotic diseases reported in humans in the territory. The territory harbors a wide variety of tropical zoonotic pathogens, including Trypanosoma cruzi, Leptospira interrogans, Toxoplasma gondii, Leishmania mexicana, Dirofilaria immitis, and Rickettsia felis. A variety of domestic, synanthropic, and wild animals act as hosts or reservoirs in the transmission cycle of the zoonotic diseases in the Yucatan Peninsula, and some spillover into human populations has also been recorded. There are still zoonotic diseases that have rarely or never been reported in humans, but it is not clear whether this is because these diseases in humans are not common, there is a lack of viable transmission cycle or there is a lack of appropriate diagnosis. It is necessary to continue monitoring vectors, animal hosts, and humans to identify risk factors for zoonotic diseases in the Yucatan Peninsula.

The objective of this study was to assess the presence of genotype 2 porcine reproductive and respiratory syndrome virus (PRRSV-2) in Baja California (Baja), as well as the standardization of the qRT-PCR technique. A cross-sectional study was conducted from 2016 to 2017 in farms from Baja. It was obtained 97 blood samples from clinically healthy, not-vaccinated boars and sows. Primers were designed and standardize, in order to perform qRT-PCR tests from the buffy coat. Every positive results were confirmed by sequence studies. It was found that 9.3 % of the samples were positive. The positive samples came from 66.6 % of the sampled regions. This study demonstrates the presence of PRRSV-2 in Baja, therefore, it is necessary to conduct epidemiological studies in order to identify the magnitude of the problem and to establish preventive and control measures.