Hepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. The protein kinase Akt plays a central role in the suppression of gluconeogenesis involving forkhead box O1 (Foxo1) and peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC-1α), and in the control of glycogen synthesis involving the glycogen synthase kinase beta (GSK3β) in the liver. It has been demonstrated that endosomal adaptor protein APPL1 interacts with Akt and blocks the association of Akt with its endogenous inhibitor, tribbles-related protein 3 (TRB3), improving the action of insulin in the liver. Here, we demonstrated that chronic exercise increased the basal levels and insulin-induced Akt serine phosphorylation in the liver of diet-induced obese mice. Endurance training was able to increase APPL1 expression and the interaction between APPL1 and Akt. Conversely, training reduced both TRB3 expression and TRB3 and Akt association. The positive effects of exercise on insulin action are reinforced by our findings that showed that trained mice presented an increase in Foxo1 phosphorylation and Foxo1/PGC-1α association, which was accompanied by a reduction in gluconeogenic gene expressions (PEPCK and G6Pase). Finally, exercised animals demonstrated increased at basal and insulin-induced GSK3β phosphorylation levels and glycogen content at 24 h after the last session of exercise. Our findings demonstrate that exercise increases insulin action, at least in part, through the enhancement of APPL1 and the reduction of TRB3 expression in the liver of obese mice, independently of weight loss.

The aim of this study was to determine the effect of 12 weeks of training on the critical velocity and maximal lactate steady state of elite swimmers. The tests to determine critical velocity and maximal lactate steady state were performed before and after 12 weeks of training. Critical velocity after 12 weeks of training was significantly higher than before training (1.4590.10 m × s (1 vs. 1.4190.11 m × s (1 ). In contrast, no significant differences in the velocity at maximal lactate steady state were observed before and after training (1.4190.10 m × s (1 vs. 1.4390.10 m × s (1 ). There was also a decrease in mean lactate concentration after 12 weeks of training. Before training, the velocity at maximal lactate steady state occurred at 100% of critical velocity, with a mean lactate concentration of 4.34 mmol × l (1 . After training, the velocity at maximal lactate steady state occurred at 98% of critical velocity, with a reduced mean lactate concentration of 3.69 mmol × l (1 . Based on these results, it would appear that 12 weeks of training was enough to promote an increase in critical velocity. Although no significant differences in the velocity at maximal lactate steady state were observed before and after training, the decrease in mean lactate concentration after training demonstrated greater efficiency of the aerobic system, leading to less wear during the tests.

ObjectiveTo investigate the effects of a specific protocol of undulatory physical resistance training on maximal strength gains in elderly type 2 diabetics.Methods The study included 48 subjects, aged between 60 and 85 years, of both genders. They were divided into two groups: Untrained Diabetic Elderly (n=19) with those who were not subjected to physical training and Trained Diabetic Elderly (n=29), with those who were subjected to undulatory physical resistance training. The participants were evaluated with several types of resistance training’s equipment before and after training protocol, by test of one maximal repetition. The subjects were trained on undulatory resistance three times per week for a period of 16 weeks. The overload used in undulatory resistance training was equivalent to 50% of one maximal repetition and 70% of one maximal repetition, alternating weekly. Statistical analysis revealed significant differences (p<0.05) between pre-test and post-test over a period of 16 weeks.Results The average gains in strength were 43.20% (knee extension), 65.00% (knee flexion), 27.80% (supine sitting machine), 31.00% (rowing sitting), 43.90% (biceps pulley), and 21.10% (triceps pulley).Conclusion Undulatory resistance training used with weekly different overloads was effective to provide significant gains in maximum strength in elderly type 2 diabetic individuals.

OBJECTIVE: The aim of the present study is to compare the values of the maximal oxygen uptake (O2 max) during two consecutive bouts in Wingate tests for arms and legs in swimmers (S) and water polo players (WP). METHODS: Sample - seven national level athletes (4 S and 3 WP), age 17,90 ± 2,14 years, body mass 71,41 ± 6,84 kg, height 176,65 ± 7,02 cm, % body fat 13,23 ± 4,18. Two Wingate bouts with 30 sec each with 3 min interval between them, for arms and legs in alternated days. Oxygen uptake: breath-by-breath using the gas analysis system K4 b² Cosmed. Statistical analysis: Wilcoxon test for dependent variables and Kolmogorov-Smirnov test for independent variables. RESULTS: The mean values found at the O2 peak (PO2), mean power (MP) and peak power (PP) for each bout of the Wingate test, for arms and legs. For Arms: PO2 = 55.16 ± 5.72 ml.kg-1.min-1, MP = 5.28 ± 0.59 watts.kg-1 and PP = 6.71 ± 0.88 watts.kg-1 got in the first bout (1st Arms) and PO2 = 60.12 ± 6.10 ml.kg-1.min-1, MP = 5.03 ± 0.40 watts.kg-1 and PP = 6.25 ± 0.51 watts.kg-1, got in the second bout (2nd Arms). For legs: PO2 = 55.66 ± 6.85 ml.kg-1.min-1, MP = 4.75 ± 1.79 watts.kg-1 and PP = 7.44 ± 1.96 watts.kg-1 got in the first bout (1st Legs) and PO2 = 62.09 ± 5.99 ml.kg-1.min-1, MP = 4.28 ± 1.47 watts.kg-1 and PP = 6.68 ± 1.63 watts.kg-1 got in the second bout (2nd Legs). DISCUSSION AND CONCLUSION: All variables studied did not present significant difference among arms and legs, as much the first as the second bout for arms for PO2 (p < 0.05). There was no difference between the PM mean values of the first and the second bout. But the mean of the second bout of legs was significant smaller than the first bout (p < 0.05). For the PP variable there was no difference among the mean values to the first and second bout as much for arms as for legs. It looks like to exist larger magnitude to O2 adjustment for arms than legs, that could be associated to specific demands to which S and WP athletes are daily submitted in their trainings