Highlights d CNTD1 associates with sites of crossing over in meiosis, colocalizing with MutLg d In the testis, CNTD1 does not interact with CDKs or with known crossover regulators d CNTD1 regulates crossing over via interactions with the replication factor C complex d CNTD1 regulates cell-cycle progression via interactions with the SCF complex

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ARTIGO DE REVISÃO A vivência da maternidade em meio à pandemiaThe experience of motherhood during the pandemic La experiencia de la maternidad en medio de la pandemia ResumoObjetivou-se evidenciar os desafios da maternidade em meio à pandemia e descrever possíveis estratégias para vivenciar esse momento sem comprometer o campo afetivo-emocional do binômio. Para isso, realizouse uma pesquisa bibliográfica em bases de dados on-line com os descritores: pandemia, maternidade, trabalho doméstico, COVID-19. Com a inserção da pandemia pela COVID-19, encontram-se mães excepcionalmente atarefadas, na medida em que realizam o trabalho remoto em casa, encarregando-se das atividades domésticas e suprindo as necessidades dos filhos, que estão em tempo contínuo na residência. A redução de interação social expande o grau de estresse, ocorre uma disfunção no padrão do sono e aumenta os níveis de cortisol na corrente sanguínea. Em decorrência disso, emergiram efeitos negativos na saúde da população, tais como: ansiedade, medo, irritabilidade, alteração de apetite, dentre outros. A vista disso, o esgotamento físico e mental evidenciado torna-se reflexo no comportamento das crianças. Verificou-se a importância de um olhar atento para mães/mulheres que vivenciam a pandemia da COVID-19. Como estratégias, evidenciam-se: solicitar ajuda de familiares; divisão de tarefas e afazeres domésticos; apoio social; atividade física ou lazer; manter contato on-line com amigos/familiares; alimentação equilibrada; partilhar relatos de experiência; estabelecer uma pausa, mesmo que breve, para descanso.

NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) is a recently developed transgene that labels each of the 118 classes of neurons in C. elegans with various combinations of four fluorescent proteins. This neuron‐type‐specific labeling helps identify neurons that could otherwise be confused with neighboring neurons. Neuron identification enables researchers to combine new data that they generate on a C. elegans neuron with existing datasets on that same neuron, such as its synaptic connections, neurotransmitters, and transcriptome. An impediment to using NeuroPAL, however, is overcoming the steep learning curve for interpreting three‐dimensional (3D) fluorescence images of crowded neural ganglia within which different neurons may be similarly colored, some neurons are only very faintly labeled, and the positions of some neurons are variable. Here, we provide protocols that allow researchers to learn to accurately identify neurons within 3D images of NeuroPAL‐labeled animals. We provide 3D reference images that illustrate NeuroPAL labeling of each body region, and additional 3D images as training exercises to learn to accurately carry out C. elegans neuron identifications. We also provide tools to annotate images in 3D, and suggest that such 3D annotated images should be the standard for documenting C. elegans neuron identifications for publication. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Using Imaris software to view and annotate images of NeuroPAL‐labeled animals in 3D Alternate Protocol: Using FIJI/ImageJ software to view and annotate images of NeuroPAL‐labeled animals in 3D Basic Protocol 2: Identifying tail neurons—an introduction to identifying neurons Basic Protocol 3: Identifying midbody neurons Basic Protocol 4: Identifying anterior head neurons Basic Protocol 5: Identifying posterior head neurons Basic Protocol 6: Identifying ventral head and retrovesicular ganglion neurons

Tossed about the tides of history, the inheritance of acquired characteristics has found a safe harbor at last in the rapidly expanding field of epigenetics. The slow pace of genetic variation and high opportunity cost associated with maintaining a diverse genetic pool are well-matched by the flexibility of epigenetic traits, which can enable low-cost exploration of phenotypic space and reactive tuning to environmental pressures. Aiding to the generation of a phenotypically plastic population, epigenetic mechanisms often provide a hotbed of innovation for countering environmental pressures, while the potential for genetic fixation can lead to strong epigenetic-genetic evolutionary synergy. At the level of cells and cellular populations, we begin this review by exploring the breadth of mechanisms for the storage and intergenerational transmission of epigenetic information, followed by a brief review of common and exotic epigenetically regulated phenotypes. We conclude by offering an in-depth coverage of recent papers centered around two critical issues: the evolvability of epigenetic traits through Baldwinian adaptive phenotypic plasticity, and the potential for synergy between epigenetic and genetic evolution.