Wounds treated with TiO 2 nanoparticles (TiO 2 -NPs) show an improvement in healing time. However, little is known about the parameters that can contribute to this result. On the other hand, the treatment of wounds with polyphenols is widely known. These compounds are found in the peel of Annona crassifl ora fruit and have antioxidant, analgesic and anti-infl ammatory properties. In this study, we evaluated the healing effect of TiO 2 nanocrystals (TiO 2 -NCs), polyphenolic fractions obtained from ethanolic extract of A. crassifl ora fruit peel (PFAC) and mix (PFAC + TiO 2 -NCs) on the parameters of wound closure, infl ammation, collagen deposition, metalloproteinase activity (MMPs) and angiogenesis. TiO 2 -NCs and PFAC have activity for wound healing, showed anti-infl ammatory action and a shorter wound closure time. These treatments also contributed to increased collagen deposition, while only treatment with TiO 2 -NCs increased MMP-2 activity, parameters essential for the migration of keratinocytes and for complete restoration of the injured tissue. The combination of PFAC + TiO 2 -NCs reduced the effectiveness of individual treatments by intensifying the infl ammatory process, in addition to delaying wound closure. We conclude that the interaction between the hydroxyl groups of PFAC polyphenols with TiO 2 -NCs may have contributed to difference in the healing activity of skin wounds.
Aims: Recombinant proteins rP2 has demonstrated biological activity in inflammation by acting on the recruitment of leukocytes and by inducing phagocytosis, also modulating the processes of angiogenesis and fibrogenesis in different experimental models. In this study we evaluated the effects of the recombinant protein rP21 from Trypanosama cruzi in cutaneous wounds.
Study Design: The wounds were induced on the back of mice and treated with rP21 at 1 µg/mL and 50 µg/mL concentration, for 3 and 7 days.
Study Location and Duration: Institute of Biomedical Sciences and Animal Breeding Network and Rodents of the Federal University of Uberlândia, between February 2015 and February 2016.
Methodology: The contraction time of wound, inflammatory cell activities (neutrophils and macrophages), angiogenesis and local collagen density were evaluated.
Sample: Wound induction was performed on 64 male BALB / c mice approximately 9 weeks old.
Results: Wounds treated with rP21 showed less closure time, in addition to exhibiting greater neutrophil activity in the initial phasis, which was reduced simultaneously with the increased macrophage activity. The rP21 also performed pro-angiogenic and pro-fibrogenic activity in this study model.
Conclusion: These results demonstrate for the first time the biological potential of rP21 in accelerating skin tissue repair.
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